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Reversibility of peroxisomal differentiation in higher plants.

高等植物中过氧化物酶体分化的可逆性。

基本信息

批准号：
15207005
负责人：
NISHIMURA Mikio
金额：
$ 31.7万
依托单位：
National Institute for Basic Biology (2004-2005)Okazaki National Research Institutes (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Peroxisomes in higher plantcells are known to differentiate in function depending on the cell type. By a comprehensive survey of peroxisomal genes in the entire Arabidopsis genome, we found 286 candidates of peroxisomal genes. They included all genes for peroxisomal enzymes reported up to date, and most of them encoded functionally unknown proteins in peroxisomes. Analyses of gene expression profiles revealed that peroxisomal differentiation is caused by the expression of specific genes that are induced in specific organs in addition to constitutively expressed genes. The clustering analyses suggested that basic functions of peroxisomewereβ-oxidation, H_2O_2 degradation, branched chain amino acid catabolism and some unknown functions, and that peroxisomesinseedlings, cotyledons, leavesand roots were differentiated by organ-specific expressed genes.In one of these mutants, apm1, the peroxisomes are long and reduced in number, apparently as a result of inhibition of division. We showed that APM1 encodes DRP3A (dynamin-related protein 3A), and that mutations in APM1/DRP3A also caused aberrant morphology of mitochondria. The transient expression analysis showed that DRP3A is associated with the cytosolicsideofperoxisomes. Thesesfindingsindicate that the same dynamin molecule is involved in peroxisomal and mitochondrialdivision in higherplants.From analyses of apm2 and apm4 mutants which are difectiveinproteintransportbyPTS1-dependent pathway, we determined that APM2 and APM4 genes encode PEROXIN13 (PEX13) and PEX12, respectively, and revealed that they are responsible for PTS2-as well as PTS1-dependent protein on the peroxisomal membrane.

已知高等植物细胞中的过氧化物酶体根据细胞类型在功能上分化。通过对拟南芥全基因组中过氧化物酶体基因的全面调查，我们发现了286个过氧化物酶体基因的候选基因。它们包括了所有的基因过氧化物酶体酶的报告日期，其中大多数编码功能未知的蛋白质过氧化物酶体。基因表达谱的分析表明，过氧化物酶体分化是由在特定器官中诱导的特定基因的表达引起的，除了组成型表达的基因。聚类分析表明，过氧化物酶体的基本功能是β-氧化、H_2O_2降解、支链氨基酸催化和一些未知功能，过氧化物酶体在幼苗、子叶、叶片和根中由器官特异性表达的基因分化，其中apm 1的过氧化物酶体变长，数量减少，这显然是分裂抑制的结果。我们发现APM 1编码DRP 3A（动力蛋白相关蛋白3A），并且APM 1/DRP 3A的突变也导致线粒体的异常形态。瞬时表达分析表明，DRP 3A与过氧化物酶体的胞浆有关。通过对突变体apm 2和apm 4的分析，我们确定了APM 2和APM 4基因分别编码PEX 13和PEX 12，并揭示了它们在过氧化物酶体膜上负责PTS 2和PTS 1依赖的蛋白。