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On the Multiplication and Antigen in Variants of the NPV OF Silkworm, Bombyx mori.,

家蚕NPV变种的增殖和抗原研究，

基本信息

批准号：
02454055
负责人：
IWASHITA Yoshimitsu
金额：
$ 4.1万
依托单位：
UTSUNOMIYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

It was found that the nuclear polyhedrosis virus of silkworm(BmNPV) exists many variants. Therefore, shape and size of particles, shape and proteins of polyhedra and structural proteins and DNA of virions were investigated comparatively between variants. 1. As with virions of passaged BmNPVs at the laboratory, the particles are rod shaped, measuring about 98-140nm wide and 350-433nm long when enveloped. 2. The virion of BmNPV is the single capsid type that a nucleocapsid is usually enveloped singly though occasionally two nucleocapsids occurred in one envelope, but La-strain is the multi-capsid type to enveloped 2 to 5 particles in enveloped. 3. Virions budding fropm midgut cells or culture cells infected with NPV showed the structural feature that nucleocapsid is bending U-shape and enveloping with plasma membrane of host cell. 4. A monoclonal antibody has been produced against virion of BmNPV I-strain by the fusion of myeloma cells. The presence of I-strain's envelope specific antigens was detected by the direct antigen negative staining method. 5. C-strain may be carried out most replicate in BmN-culture cells. 6. The size of restriction fragements of viral DNA of 4 strains (T, Ta, C and 54N) were determined with reference to phage lamda DNA digested with HindIII, EcoRI, BanHI, KpnI and SmaI. Similarities of base sequences were found between C and 54N, and between T and Ta strains, respectively. 7. Polyhedral proteins of T, Ta, C and 54N strains were electerphoresed on SDS- PAGE method. The T-strain may be attributed to the deletion of 57.6KDa polypeptied of the polyhedral protein.

家蚕核型多角体病毒(BmNPV)存在多种变种。因此，对不同变异体的颗粒形状和大小、多角体的形状和蛋白质以及病毒粒子的结构蛋白和DNA进行了比较研究。1.与实验室传代的BmNPV病毒粒子一样，这些粒子呈杆状，被包裹时宽约98-140 nm，长350-433 nm。2.BmNPV的病毒粒子是单衣壳类型，即一个核衣壳通常是单个包膜的，但偶尔会在一个包膜中出现两个核衣壳，而La株是多衣壳类型，包膜2-5个颗粒。3.从感染NPV的中肠细胞或培养细胞中萌发的病毒粒子呈现出核衣壳呈U形弯曲，包裹在宿主细胞质膜上的结构特征。4.通过骨髓瘤细胞融合，制备了抗BmNPV I株病毒粒子的单抗。用直接抗原负染法检测I株包膜特异性抗原的存在。5.在BMN培养的细胞中，C-株的复制能力最强。6.以HindIII、EcoRI、BanHI、KpnI和SmaI酶切噬菌体Lamda DNA为参照，测定了T、Ta、C和54N四株病毒DNA的限制性片段大小。C株与54N株之间以及T株与Ta株之间的碱基序列具有相似性。7.对T、Ta、C和54N菌株的多角体蛋白进行了SDS-PAGE分析。该T株可能与多面体蛋白57.6 KDa多肽缺失有关。