The investigation on the relation between the glutathione metabolism and liver atrophy.

肝萎缩过程中胱甘肽代谢的研究。这项研究的重点是谷胱甘肽代谢的变化，它被认为是一种清道夫，在肝脏反应的结果。（方法）将Wistar大鼠分为两组，实验组；上颈静脉（SMV）夹持30min后取下。我;假手术组；简单剖腹手术。酶促法测定各组肝组织、肝上下腔静脉（IVC）和胆汁中GSH和GSSG，酶促法测定腺嘌呤核苷，并用阿特金斯法计算能量电荷（EC）。（结果）SMV夹紧和再灌注后肝组织中GSH和GSSG水平无变化，而肝上IVC中GSH和GSSG水平在SHV夹紧10 min后10 min较假手术组明显升高（GSH约2倍，GSSG超过3倍），且这种高水平谷胱甘肽维持至SMV夹紧30 min后120 min。二值GSH和GSSG水平在SMV钳夹后下降，再灌注后升高。SMV钳夹后肝脏ATP和EC下降，再灌注后10分钟恢复。认为肝脏GSII/GSSG比值是通过释放肝组织氧化应激产生的GSSG来维持的。胆汁中GSSG水平的变化与ATP和EC的变化相关，被认为依赖于ATP。scrum中GSSG水平的变化先于转氨酶水平的变化。在此基础上，研究了大鼠肝脏代偿期细胞因子（tnf - α、il - β）与高代谢状态的关系。通过系统给药tnf - α和il - β，恢复肝脏线粒体氧化还原状态，抑制生酮。能源需求增加导致EC下降。

Investigation on the giutathione metaboism during the process for liver atrophy.This research was focused upon the changes of glutathione metabolism, which is considered to work as a scavenger, in consequence of hepatic repercusion.1. (Method)Wistar rats were divided to two groups, experimental group ; superior nescntcric vein (SMV) was clamped for 30 min and then deciampcd. I ; Sham operation group ; simple laparotomy was donc. In each group, GSH and GSSG were measured enzymatically in liver tissue, scrum from supra hepatic inferior vena cava (IVC) and bile sample, and adenine nucleotistes were measured enzymatically and energy charge (EC)was calculated by mean of Atkins.(Results)GSH and GSSG in liver tissue after SMV clamping and reperfusion did not change, while GSH and GSSG level in supra hepatic IVC increased signifcantly (about 2 times in GSH and over 3 times in GSSG) compared with Sham operation group 10 min after 10 min SHV clamp, and these high level of glutathiones were kept until 120 min after SMV clamp for 30 min. Binary GSH and GSSG level decreased consequently after SMV clamp and reelevated after reperfusion. Hepatic ATP and EC decreased in consequence of SMV clamp and then recovered 10 min after reperfusion.(Discussion).It is considered that the hepatic GSII/GSSG ratio is maintained by releasing GSSG generated by oxidant stress from liver tissue.The changes of GSSG level in bile is correlated to the changes of ATP and EC, and it is considered to depend on ATP. The change of GSSG level in scrum precede those of transaminases.2. In relation to these ressearch, the relation between cytokines (TNF-alpha, ILl-beta) and hypermetabolic state in compernsatory phase of liver were investigated in rats. By systematic administration of TNF-alpha and ILl-beta, hepatic mitochondrial redox state was restored and ketogenesis was suppresscd. EC decreased by increased energy demand.