Behavior of Hybrid Materials Compounded with Culture Cells and New Biomaterials in Vivo

我们一直在研究骨诱导新型杂交材料的开发。在这个时候，我们产生了一个混合材料复合成纤维细胞，可以分化成成骨细胞和/或成牙骨质细胞和多孔羟基磷灰石。从切牙根部获得的牙周膜韧带细胞在37 ℃下培养。C在α-MEM加10%胎牛血清中。实验动物为日本白色家兔。在离体兔下颌骨上分别制作两个邻近不同磨牙根面的人工骨缺损。将新型混合材料颗粒置于一个骨缺损中，另一个骨缺损填充多孔羟基磷灰石颗粒作为对照。手术后3周和6周，处死动物，固定在10%福尔马林中，在5%三氯乙酸中脱矿物质，并以常规方式包埋在石蜡中。取6 μ m厚连续切片，H. E.并使用光学显微镜进行组织学观察。术后3周的结果显示，实验区和对照区之间没有观察到特征性差异。在这两个地方，吞噬细胞像一个多核巨细胞附着在肉芽表面，新骨开始从相邻的下颌骨形成。颗粒部分被新骨包围。术后6周的观察结果表明，在新的混合材料表面上识别出一些类骨质，如钙化物质。在这些区域，细胞垂直侵入颗粒。另一方面，在对照区未观察到这些发现，颗粒周围有纤维结缔组织。这些钙化物可能来源于牙周膜细胞。但新的钙化物质是否是由牙周膜细胞诱导产生的，还有待进一步研究才能明确。

We have been studying on the development of osteoinductive new hybrid materials. In this time we produced a hybrid material compounded with fibroblasts which may differentiate into osteoblast and or cementoblast and porous hydroxyapatite. Periodontal ligament cells obtained from incisor roots, were cultured at 37^。C in alpha-MEM plus 10% fetal calf serum. The experimental animals were Japanese White Rabbits. Artificial separate two bone defects adjacent to different molar root surfaces were prepared in the mandible of rabbit. The new hybrid material granules were placed in the one bone defect and the other one was filled with porous hydroxyapatite granules as control. Three and 6 weeks after the operation, the animals were sacrificed, fixed in 10% formalin, demineralized in 5% trichloroacetic acid and embedded in paraffin in the usual manner. Then the serial sections 6 mum in thick were obtained, stained with H. E. and observed histologically using light microscopy. The findings at 3 weeks after the operation showed that a characteristic differenece could not be observed between experimental and control areas. On both places, phagocytes like a polynuclear giant cell attached to the granulor surfaces, and new bone began to form from the adjacent mandibular bone. The granules were partly surrounded with new bone. The findings at 6 weeks after the operation showed that some osteoid like a calcified material was recognized on the surface of new hybrid materials. On these areas the cells invaded perpendicularly into the granules. On the other hand, on the control areas these findings were not observed, and the granules were surrounded with fibrous connective tissues. These calcified materials formed on the granule surfaces may be considered to come from the periodontal ligament cells. But it would be clarified in further studies if the new calcified materials are induced by the periodontal ligament cells.