Behavior of Hybrid Materials Compounded with Culture Cells and New Biomaterials in Vivo

我们一直在研究骨诱导新型杂化材料的开发。本研究制备了一种复合纤维母细胞材料，该材料可分化为成骨细胞和成水泥细胞和多孔羟基磷灰石。从门牙根中获得的牙周韧带细胞在37℃培养。α - mem中的C加上10%的胎牛血清。实验动物为日本大白兔。在兔下颌骨不同牙根面相邻处制备了人工分离的两个骨缺损。将新型杂化材料颗粒置入一个骨缺损内，另一个骨缺损内填充多孔羟基磷灰石颗粒作为对照。术后3周、6周处死，10%福尔马林固定，5%三氯乙酸脱矿，石蜡包埋。取厚的连续切片6片，进行he染色，光镜下观察组织学变化。术后3周的结果显示，试验区和控制区之间没有观察到特征差异。在这两个地方，吞噬细胞像多核巨细胞一样附着在颗粒表面，新骨开始从邻近的下颌骨形成。颗粒部分被新骨包围。术后6周发现在新混合材料表面识别出一些类骨样钙化物质。在这些区域，细胞垂直侵入颗粒。另一方面，在对照区没有观察到这些发现，颗粒被纤维结缔组织包围。这些在颗粒表面形成的钙化物质可以认为来自牙周韧带细胞。但新钙化材料是否由牙周韧带细胞诱导，有待进一步研究。

We have been studying on the development of osteoinductive new hybrid materials. In this time we produced a hybrid material compounded with fibroblasts which may differentiate into osteoblast and or cementoblast and porous hydroxyapatite. Periodontal ligament cells obtained from incisor roots, were cultured at 37^。C in alpha-MEM plus 10% fetal calf serum. The experimental animals were Japanese White Rabbits. Artificial separate two bone defects adjacent to different molar root surfaces were prepared in the mandible of rabbit. The new hybrid material granules were placed in the one bone defect and the other one was filled with porous hydroxyapatite granules as control. Three and 6 weeks after the operation, the animals were sacrificed, fixed in 10% formalin, demineralized in 5% trichloroacetic acid and embedded in paraffin in the usual manner. Then the serial sections 6 mum in thick were obtained, stained with H. E. and observed histologically using light microscopy. The findings at 3 weeks after the operation showed that a characteristic differenece could not be observed between experimental and control areas. On both places, phagocytes like a polynuclear giant cell attached to the granulor surfaces, and new bone began to form from the adjacent mandibular bone. The granules were partly surrounded with new bone. The findings at 6 weeks after the operation showed that some osteoid like a calcified material was recognized on the surface of new hybrid materials. On these areas the cells invaded perpendicularly into the granules. On the other hand, on the control areas these findings were not observed, and the granules were surrounded with fibrous connective tissues. These calcified materials formed on the granule surfaces may be considered to come from the periodontal ligament cells. But it would be clarified in further studies if the new calcified materials are induced by the periodontal ligament cells.