Behavior of Hybrid Materials Compounded with Culture Cells and New Biomaterials in Vivo

培养细胞与新型生物材料复合的杂化材料在体内的行为

• 批准号: 02454442
• 负责人: ARAI Takashi
• 金额: $ 4.42万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454442/
• 关键词: Periodontal ligament cell; Porous Hydroxyapatite; Hybrid Material; Calcification

We have been studying on the development of osteoinductive new hybrid materials. In this time we produced a hybrid material compounded with fibroblasts which may differentiate into osteoblast and or cementoblast and porous hydroxyapatite. Periodontal ligament cells obtained from incisor roots, were cultured at 37^。C in alpha-MEM plus 10% fetal calf serum. The experimental animals were Japanese White Rabbits. Artificial separate two bone defects adjacent to different molar root surfaces were prepared in the mandible of rabbit. The new hybrid material granules were placed in the one bone defect and the other one was filled with porous hydroxyapatite granules as control. Three and 6 weeks after the operation, the animals were sacrificed, fixed in 10% formalin, demineralized in 5% trichloroacetic acid and embedded in paraffin in the usual manner. Then the serial sections 6 mum in thick were obtained, stained with H. E. and observed histologically using light microscopy. The findings at 3 weeks after the operation showed that a characteristic differenece could not be observed between experimental and control areas. On both places, phagocytes like a polynuclear giant cell attached to the granulor surfaces, and new bone began to form from the adjacent mandibular bone. The granules were partly surrounded with new bone. The findings at 6 weeks after the operation showed that some osteoid like a calcified material was recognized on the surface of new hybrid materials. On these areas the cells invaded perpendicularly into the granules. On the other hand, on the control areas these findings were not observed, and the granules were surrounded with fibrous connective tissues. These calcified materials formed on the granule surfaces may be considered to come from the periodontal ligament cells. But it would be clarified in further studies if the new calcified materials are induced by the periodontal ligament cells.

我们一直在研究开发诱导成骨的新型杂化材料。在此期间，我们生产了一种与成纤维细胞复合的杂化材料，可以分化为成骨细胞和/或成牙骨质细胞和多孔羟基磷灰石。从切牙根部获取牙周膜细胞，37℃培养。α-MEM+10%胎牛血清。实验动物为日本大白兔。在兔下颌骨不同磨牙根面附近制备人工分离的两个骨缺损区。将新型杂化材料颗粒植入其中一处骨缺损处，另一处植入多孔羟基磷灰石颗粒作为对照。术后3周、6周处死动物，10%福尔马林固定，5%三氯乙酸脱钙，常规石蜡包埋。取6微米厚的连续切片，HE染色，光镜下观察。术后3周的检查结果显示，实验区和对照区之间没有明显的差异。在两处，吞噬细胞类似多核巨细胞附着在颗粒表面，并从邻近的下颌骨开始形成新骨。颗粒周围部分被新生骨包围。术后6周的结果显示，新型混杂材料表面可见类骨样钙化物质。在这些区域，细胞垂直侵入颗粒。另一方面，在对照区域没有观察到这些发现，颗粒周围有纤维结缔组织。这些颗粒表面形成的钙化物质可能来自牙周膜细胞。但如果新的钙化物质是由牙周韧带细胞诱导的，将在进一步的研究中得到澄清。