Study of the neutrophil functions in pregnancy

妊娠期中性粒细胞功能的研究

• 批准号: 05671357
• 负责人: ARAI Takashi
• 金额: $ 1.41万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671357/
• 关键词: Pregnancy; Neutrophil; Toxemia of pregnancy; Leukotriene B4; Arachidonic acid; Phospholipid; Neutrophil elastase; Chemotaxis; 好中球; エラスターゼ; 走化性; 性ステロイド; インターロイキン1β; 正常妊婦; ロイコトリエンB_4; カルシウムイオノフォア

(1) Calcium ionophore-induced leukotriene B4 release from neutrophils is suppressed in 2nd and 3rd trimester of normal pregnancy but not in toxemic pregnancy.(2) Released amounts of leukotriene B4 from neutrophils of toxemic patients are at identical level with nonpregnant women and significantlly correlated with birth weights and its z scores at each gestational age.(3) Arachidonic acid content and mole% in phospholipids of neutrophils decreased with gestational weeks.(4) Ratio of unsaturated fatty acid/saturated fatty acid decreased with gestational weeks.(5) These findings suggest that the neutrophil activation is suppressed in normal pregnancy in terms of leukotriene B4 release and of the fluidity of the membrane and that disorders of these mechanism seem to induce toxemia of pregnancy.(6) Elastase contents of neutrophils and PMA-stimulated elastase release from neutrophil are not different among pregnant, non-poregnant woman and men.

(1)钙离子载体诱导的中性粒细胞释放白三烯B4在正常妊娠的第2和第3个月受到抑制，但在毒血症妊娠中没有。(2)中毒患者中性粒细胞释放的白三烯B_4量与非孕妇女相当，并与各胎龄的出生体重及其Z值显著相关。(3)中性粒细胞磷脂中花生四烯酸含量和摩尔%随孕周的增加而降低。(4)不饱和脂肪酸/饱和脂肪酸比值随孕周增加而降低。(5)这些研究结果表明，中性粒细胞的激活抑制在正常妊娠的白三烯B4的释放和膜的流动性，这些机制的障碍似乎诱导妊娠毒血症。(6)中性粒细胞的弹性蛋白酶含量和PMA刺激的中性粒细胞释放的弹性蛋白酶在妊娠、非妊娠妇女和男性之间没有差异。

项目成果

Toshihiro IMAI and Takashi ARAI: "Quantitative changes in Leukotriene B_4 Relases in neutrophilic leukocytes activated by Ca ionophore during pregnancy-Normal pregnancy and Preeclampsia-" Acta Obstetrica et Gynaecologica Japonica. 48. (1996)

Toshihiro IMAI 和 Takashi ARAI：“妊娠期间 Ca 离子载体激活的中性粒细胞中白三烯 B_4 释放的定量变化 - 正常妊娠和先兆子痫 -” Acta Obstetrica et Gynaecologica Japonica。

今井敏啓,新居隆: "妊娠経過と好中球ロイコトリエンB4-正常妊娠と妊娠中毒症における量的変動-" 日本産科婦人科学会雑誌. (1996)

Toshihiro Imai、Takashi Arai：“妊娠过程和中性粒细胞白三烯 B4 - 正常妊娠和先兆子痫的数量变化”日本妇产科学会杂志（1996 年）。