Biochemical degradation of phenols by yeast
酵母对酚类物质的生化降解
基本信息
- 批准号:03454031
- 负责人:
- 金额:$ 3.9万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Biodegradation of phenol and monochlorophenols (CPs) by a yeast strain of Rhodotorula glutinis was exmined. The strain completely degraded 5 mM of phenol and utilized phenol as a sole carbon source. The strain may degrade phenol by the "ortho" type of ring fission because muconolactone was observed in the cultured broth. 3-Chlorophenol (3-CP) and 4-chlorophenol (4-CP) were well degraded and stoichiometric release of chloride ion was observed, but degradation of 2-Chlorophenol was low with few amount of chloride ion. Biodegradability of 3-CP and 4-CP was increased by the addition of phenol or CPs to the medium at the cell cultivation. Since 4-chlorophenol and maleylacetic acid were determined as metabolites of 3-CP and 4-CP by GC/Ms analysis, dechlorination may take place between the formation of 4-chlorophenol and maleylacetic acid.Biochemical degradation of phenols by immobilized yeast cells was examined. Repeated batch cultures (10 days) for the degradation of phenol were performed f … More or comparing three kinds of beads, made from cellulose, chitosan and sintered glass. In the initial incubation period, cellulose beads as a carrier had higher yeast cells immobilizing capacity and had higher removal of TOC and phenol(%) than chitosan and sintered glass beads. The numbers of immobilized yeast cells with cellulose beads finally reached 2.5 X 10^8 cells/ml-beads. This result was almost the same as with chitosan and sintered glass beads. Continuous culture was performed with cellulose beads. Phenol (5 mM) and 3- and 4-CP (0.05 mM) was degraded stably for 100 days in this system.Identification of beta-ketoadipic acid in muconolactone and protocatechuic acid metabolites by the crude cell extract of yeast Rhodotorula rubra was carried out using methoxime-trimethylsilyl (MO-TMS) derivative to prevent enol ether formation. beta-Ketoadipic acid was converted successfully to MO-TMS derivative, and enol ether formation was not observed. Mass spectra of two isomers of MO-TMS derivative of beta-ketoadipic acid resembled each other in their fragmentation pattern ; they had sufficient intensities of molecular ion at m/z 333, and other characteristic ions. Less
研究了粘红酵母对苯酚和单氯酚的生物降解作用。菌株完全降解5 mM的苯酚,并利用苯酚作为唯一的碳源。该菌株可通过“邻位”型环裂变降解苯酚,因为在培养的肉汤中观察到黏液内酯。3-氯酚(3-CP)和4-氯酚(4-CP)降解效果良好,氯离子有化学计量释放,但2-氯酚的降解效果较差,氯离子含量少。在细胞培养时,在培养基中添加苯酚或CPs可提高3-CP和4-CP的生物降解性。由于GC/Ms分析确定4-氯苯酚和马来酰乙酸是3-CP和4-CP的代谢物,因此在4-氯苯酚和马来酰乙酸的生成过程中可能发生脱氯作用。研究了固定化酵母细胞对酚类物质的生化降解。对纤维素、壳聚糖和烧结玻璃制成的三种微球进行了反复分批培养(10 d),对苯酚的降解进行了比较。在培养初期,纤维素微球作为载体比壳聚糖和烧结玻璃微球具有更高的酵母细胞固定化能力,对TOC和苯酚的去除率(%)也更高。用纤维素球固定酵母细胞的数量最终达到2.5 × 10^8个细胞/ml-beads。这一结果与壳聚糖和烧结玻璃微珠几乎相同。纤维素珠连续培养。苯酚(5 mM)和3-和4-CP (0.05 mM)在该系统中稳定降解100天。采用甲氧肟-三甲基硅基(MO-TMS)衍生物对酵母红酵母粗细胞提取物黏液内酯和原儿茶酸代谢产物中β -酮己二酸进行了鉴定,以防止烯醇醚的形成。β -酮己二酸成功转化为MO-TMS衍生物,未观察到烯醇醚的形成。-酮己酸MO-TMS衍生物的两个同分异构体的质谱在断裂模式上相似;它们在m/ z333处具有足够的分子离子强度和其他特征离子。少
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
平山けい子 他: "β-ケトアジピン酸のメトキシム-トリメチルシリル誘導体によるGC/MS分析" 水処理技術. in press. (1994)
Keiko Hirayama 等人:“使用甲肟-三甲基甲硅烷基衍生物对 β-酮己二酸进行 GC/MS 分析”水处理技术 (1994)。
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- 影响因子:0
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- 通讯作者:
Keiko KATAYAMA-HIRAYAMA, Shusaku TOBITA and Kimiaki HIRAYAMA: "Biodegration of phenol and monochlorophenols by yeast Rhodotorula glutinis" Water Science and Technoloty. (in press).
Keiko KATAYAMA-HIRAYAMA、Shusaku TOBITA 和 Kimiaki HIRAYAMA:“酵母红酵母对苯酚和一氯酚的生物降解”水科学与技术。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
平山 けい子: "酵母によるフェノ-ル類の分解(予定)" 土木学会第47回年次学術講演会. 47. (1992)
平山惠子:“酵母分解苯酚(计划)”第 47 届日本土木工程学会学术年会 47。(1992 年)
- DOI:
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- 影响因子:0
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平山けい子,飛田修作,平山公明: "β-ケトアジピン酸のメトキシム-トリメチルシリル誘導体によるGC/MS分析" 水処理技術. (in press). (1994)
Keiko Hirayama、Shusaku Tobita、Kimiaki Hirayama:“使用甲氧肟-三甲基甲硅烷基衍生物对 β-酮己二酸进行 GC/MS 分析”(出版中)。
- DOI:
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- 影响因子:0
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平山けい子 他: "Rhodotorula属酵母によるフェノールおよびモノクロロフェノールの分解" 水処理技術. 33. 551-555 (1992)
Keiko Hirayama 等人:“红酵母属酵母对苯酚和一氯苯酚的分解”水处理技术 33. 551-555 (1992)。
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KATAYAMA Keiko其他文献
KATAYAMA Keiko的其他文献
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{{ truncateString('KATAYAMA Keiko', 18)}}的其他基金
INVESTIGATION OF PRESENT CONDITION OF HBV & HCV INFECTION FOR CONTROL AND PREVENTION OF HBV & HCV INFECTION IN HEMODIALYSIS PATIENTS
血液透析患者乙型肝炎、丙型肝炎感染现状调查,控制和预防乙型肝炎、丙型肝炎感染
- 批准号:
12670357 - 财政年份:2000
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Biological treatment of dissolved organic matter by antarctic psychrophilic yeast cells
南极嗜冷酵母细胞对溶解有机物的生物处理
- 批准号:
06650603 - 财政年份:1994
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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