Characterization of novel dwarfing substances A-2alpha and beta, and their application to horticulture

为了鉴定在矮豌豆cv中起光抑制作用的新型生长抑制剂A-2 α和β。为了找到在园艺上的应用，我们尝试从大田生长的进步豌豆苗中分离出它们。虽然a -2的纯分离由于其极端不稳定而未能成功，但获得了许多对豌豆芽的大规模提取和纯化以及培养和储存有用的信息，如下：无论培养季节、培养周期如何，也无论芽的形状如何，主芽和侧芽衍生的芽都可以作为提取材料；在收获后立即冷冻芽是必要的，并且在-20℃的温度下储存可以使抑制剂的含量保持多年不变。此外，我们成功地在反相HPLC图谱上定位了A-2 α， β和α， A-3和A-1-3's，这对分离过程中抑制剂的检测和定量有很大的帮助。我们发现了新的抑制剂，命名为A-1-3 α和β，并成功地鉴定了它们的HPLC峰。A-1-3 α和β是在溶液中相互变化的异构体。

In order to characterize novel growth inhibitors A-2 alpha and beta responsible for light growth inhibition in the dwarf pea cv. Progress No. 9 and to find an application to horticulture, their isolation was attempted from Progress pea shoots grown in the field. Although an pure isolation of A-2's was not successful because of their extreme instability, many pieces of information useful for a large-scale extraction and purification as well as for culture and storage of pea shoots were obtained as follows: irrespective of the culture seasons, culture periods, and hence of the different shapes of shoots, the main shoot as well as the shoots derived from lateral buds serve as extraction materials; freezing shoots immediately after harvesting is necessary, and storage at -20ﾟ C keeps the inhibitor content unchanged for years. Also, we succeeded in locating A-2 alpha,beta and alpha and A-3's as well as A-1-3's on reverse phase HPLC chromatograms, which serve greatly for detection and quantification of the inhibitors in isolation processes. We found novel inhibitors named A-1-3 alpha and beta, and succeeded in identifying their HPLC peaks. A-1-3 alpha and beta were found to be isomers which change from one another in solution.