Characterization of novel dwarfing substances A-2alpha and beta, and their application to horticulture

新型矮化物质A-2α和β的表征及其在园艺中的应用

• 批准号: 03454048
• 负责人: HASHIMOTO Tohru
• 金额: $ 2.11万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454048/
• 关键词: Dwarf pea; GC; Growth inhibitors; HPLC; Pisum sativum; A-1-3; Isomers; 大量抽出処理; ガスクロマトグラフ

In order to characterize novel growth inhibitors A-2 alpha and beta responsible for light growth inhibition in the dwarf pea cv. Progress No. 9 and to find an application to horticulture, their isolation was attempted from Progress pea shoots grown in the field. Although an pure isolation of A-2's was not successful because of their extreme instability, many pieces of information useful for a large-scale extraction and purification as well as for culture and storage of pea shoots were obtained as follows: irrespective of the culture seasons, culture periods, and hence of the different shapes of shoots, the main shoot as well as the shoots derived from lateral buds serve as extraction materials; freezing shoots immediately after harvesting is necessary, and storage at -20ﾟ C keeps the inhibitor content unchanged for years. Also, we succeeded in locating A-2 alpha,beta and alpha and A-3's as well as A-1-3's on reverse phase HPLC chromatograms, which serve greatly for detection and quantification of the inhibitors in isolation processes. We found novel inhibitors named A-1-3 alpha and beta, and succeeded in identifying their HPLC peaks. A-1-3 alpha and beta were found to be isomers which change from one another in solution.

为了表征新的生长抑制剂A-2 α和β负责轻生长抑制矮豌豆品种。Progress No.9的分离物，并发现其在园艺中的应用，尝试从生长在田间的Progress pea芽中分离它们。虽然A-2的纯分离由于其极不稳定性而不成功，但获得了许多对大规模提取和纯化以及培养和储存豌豆苗有用的信息，如下所示：不考虑培养季节、培养周期以及由此产生的芽的不同形状，将主芽以及源自侧芽的芽用作提取材料;收获后立即冷冻嫩枝是必要的，在-20 ° C下储存可使抑制剂含量保持多年不变。此外，我们成功地定位A-2 α，β和α和A-3的以及A-1-3的反相HPLC色谱图，这大大有助于检测和定量的抑制剂在分离过程中。我们发现了名为A-1-3 α和β的新型抑制剂，并成功地鉴定了它们的HPLC峰。发现A-1-3 α和β是在溶液中彼此变化的异构体。

项目成果

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Hashimoto,T.: "Growth inhibitors A-2α and A-2β,and red light-induced growth inhibition in dwarf pea." Progress in Plant Growth Regulation. /. 312-318 (1991)

Hashimoto, T.：“生长抑制剂 A-2α 和 A-2β，以及红光诱导的矮豌豆生长抑制。”植物生长调节进展 /。

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