Morphological analysisi of bacterial capsule by freeze-substitution technique.

通过冷冻替代技术对细菌荚膜进行形态学分析。

• 批准号: 03454183
• 负责人: AMAKO Kazunobu
• 金额: $ 3.9万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454183/
• 关键词: Capsule; Capsular swelling; Freeze-substitution; Electron microscopy; Klebsiella pneumoniae; 肺炎桿〓; 凍結法

The fine structures of the capsules of Klebsiella pneumoniae and Staphylococcus aureus were examined with new fixation technique for electron microscopy and following new findings were obtained.1. The capsules were consisted of the layer of the fine fibers which were arranged radially on the cell surface.2. The capsule of S. aureus did not cover the entire cell surface. Some part of the surface of encapsulated strains were exposed to outside3. The anti-O serum penetrated the capsule layer of Kl. pneumoniae, but the anti-teichoic acid serum did not penetrate the layer of S. aureus. The capsule of S. aureus seemed to have a function of the barrier for the penetration of high molecular materials.4. By repeated washing of the bacteria the capsule swelled and lost of its electron density.5. The anti-capsule serum induced the swelling of the capsular layer.6. Fab or half molecule of IgG could also induce the swelling. This showed that even monoreactive antibody could induce the swelling.

采用新的电镜固定技术对肺炎克雷伯菌和金黄色葡萄球菌荚膜的微细结构进行了观察，获得了以下新发现.囊膜由细胞表面呈放射状排列的一层细纤维构成. S.金黄色葡萄球菌没有覆盖整个细胞表面。被包裹的菌株的部分表面暴露于外部3。抗O血清穿透Kl的囊层。pneumoniae，但抗磷壁酸血清不能穿透S.金黄色。S.金黄色葡萄球菌似乎对高分子物质的渗透具有屏障作用.通过反复洗涤细菌，胶囊膨胀并失去其电子密度。抗囊膜血清引起囊膜肿胀. Fab或半分子IgG也可引起肿胀。这表明，即使是单反应性抗体也可以诱导肿胀。

项目成果

Takeshi Arizono: "Distribution of capsular materials on the cell wall surface of strain Smith diffuse of Staphylococcus aureus." Journal of Bacteriology. 173. 4333-4340 (1991)

Takeshi Arizono：“金黄色葡萄球菌史密斯扩散菌株细胞壁表面上荚膜材料的分布。”

天児 和暢: "細菌のきよう膜" 電子顕微鏡. 26. 10-15 (1991)

Kazunobu Amago：“细菌膜”电子显微镜。26. 10-15 (1991)

Akiko Umeda: "Localigation of Peptidogly can an teichoic aeid on the cell wall surface of Staphylococcus ausew as detesmined by immuro election microscopy" Taurnal of Electwr Wicroscopy. 41. 46-52 (1992)

Akiko Umeda：“通过免疫电子显微镜测定，肽聚糖的定位可以在葡萄球菌细胞壁表面上形成磷壁酸”Taurnal of Electwr Wicroscopy。

Yuko Meno: "The surface hydrophobicity and avirulent characters of an encapsulated strain of Klebsiella pneumoniae." Microbiology and Immunology. 35. 841-848 (1991)

Yuko Meno：“肺炎克雷伯菌封装菌株的表面疏水性和无毒特性。”

Yoko Meno: "The surface hydrophobicity and arirulent characters of an encapsulated strain of Klebsiella pneumoniac" Microbiology and Immunology. 35. 841-848 (1991)

Yoko Meno：“肺炎克雷伯氏菌封装菌株的表面疏水性和腐蚀性特征”微生物学和免疫学。