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The group studies on the structural and functional roles of the bacterial surfaces in the infections.

该小组研究细菌表面在感染中的结构和功能作用。

基本信息

批准号：
60304052
负责人：
AMAKO Kazunobu
金额：
$ 4.86万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

The works of the member in this project can be categorized into three different experimental groups, the group on the colonization factor, on the biological activity of cell surface materiales and on the invasing mechanisms. The experimental results of these groups in these three years are summerized as follow.The group of the colonization factors: From several species of bacteria fimbrial colonization factors were isolated, purified and the chemical compositions were analysed. In E. coli a new fimbriae called CFA III was isolated and ch aracterized. In S.marcescens a gene for MR fimbria was cloned and expressed in E. coli. Its DNA sequence analysis showed that the sequence of amino acids at the N-terminal portion of the fimbrial subunit was almost the same as those of the other morphologically indistinguishable fimbria of E. coli. As a colonization factor for Vibrio cholerae the roles of hemagglution on the adherece on the intestinal mucosa of human was demonstrated.The group of biolo … More gical activity of suface materiales: As one of the biological effects of the cell wall components, MDP,a muramyl dipeptide, exserted a stimulating effect against the toxic effect of the endotoxin. Mice pretreated with MDP showed an anaphylactic reaction when a small amount of LPS was administered intravacularly. The mechanisms of this phenomenon is remained to be elucidated. The role of coagulase and protein A on the infection of Staphylococcus aureus are investigate using the deficient mutant of these cell wall components. Coagulase is more closely related to the virulence of this bacteria.The group of the invasiveness: For the analysis of the invasivenes of Shigella spp. the monoclonal antibodies for the cell surface of Sh. sonnei was produced. Some of the clones were reacted only with phase I strains of this bacteria. These antibodies might be usefull for the anlysis of invasive factors of this bacteria. The morphological and biological studies of the regular surface array ,an protective barrier on the cell surface, were carried out on Clostridium spp.. Less

该项目成员的工作可分为三个不同的实验组，即定植因子组、细胞表面物质的生物活性组和侵袭机制组。三年来的实验结果如下：定殖因子组：从几种细菌菌毛中分离、纯化定殖因子，并对其化学成分进行分析。在大肠从大肠杆菌中分离到一种新的菌毛，命名为CFA Ⅲ。在粘质沙雷氏菌中克隆了MR菌毛基因，并在大肠杆菌中表达。杆菌DNA序列分析表明，该菌毛亚基N端的氨基酸序列与其它形态上难以区分的E.杆菌血凝素作为霍乱弧菌的定植因子，对霍乱弧菌在人肠粘膜上的粘附作用进行了研究，并对血凝素的生物学特性进行了初步探讨。 ...更多信息表面物质的生物活性：MDP是胞壁酰二肽，作为细胞壁成分的生物效应之一，对内毒素的毒性作用具有刺激作用。MDP预处理的小鼠在血管内注射少量LPS时表现出过敏反应。这一现象的机制仍有待阐明。利用凝固酶和蛋白A的缺陷突变体研究了凝固酶和蛋白A在金黄色葡萄球菌感染中的作用。凝固酶与志贺菌的毒力关系更为密切。Sh.索尼制作了。一些克隆仅与该细菌的I相菌株反应。这些抗体可用于分析该菌的侵袭因子。对梭状芽孢杆菌（Clostridium spp.）细胞表面的保护屏障--规则表面阵列进行了形态学和生物学研究。少