Studies on immunoglobulin class switch recombination

免疫球蛋白类别转换重组的研究

• 批准号: 03454196
• 负责人: YAMAGISHI Hideo
• 金额: $ 3.9万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454196/
• 关键词: Immunoglobulin gene; Class switch; LPS and TGF-beta; IgA switch induction; Circular DNA; Salpha region; Successive recombination; IL-2 stimulated proliferation; TGF-βとIL-2; 細胞増殖; IgAクラススイッチ; Sμ; Sγ_3組換え; 組換え点の均一化; クラススイッチ組換え; 完全塩基配列; スイッチ組

We have characterized extrachromosomal circular DNAs from adult mouse spleen cells that were induced to switch to immunoglobulin A (IgA) with bacterial lipopolysaccharide (LPS) and transforming growth factor beta (TGF-beta), and identified breakpoints of switch recombinants. Our results support the hypothesis that TGF-beta increases the frequency of switch recombination events to IgA and that the switch recombination to IgA often proceeds by siccessive recombination. However, TGF-beta induced class switch recombination was modified during the cell proliferation restored by IL-2 stimulation. All the breakpoints of the switch alpha region were located within the consensus sequence subsets of the 4.2 kb repetitive region completed in this study.

我们对成年小鼠脾细胞的染色体外环状 DNA 进行了表征，这些细胞被细菌脂多糖 (LPS) 和转化生长因子 β (TGF-β) 诱导转换为免疫球蛋白 A (IgA)，并鉴定了转换重组体的断点。我们的结果支持这样的假设：TGF-β增加了IgA转换重组事件的频率，并且IgA转换重组通常通过连续重组进行。然而，TGF-β 诱导的类别转换重组在 IL-2 刺激恢复的细胞增殖过程中发生了改变。开关 α 区域的所有断点均位于本研究完成的 4.2 kb 重复区域的共有序列子集中。

项目成果

山岸秀夫: "抗体のクラススイッチと環状DNA" 代謝 増刊号 「免疫‘91」. 28. 123-129 (1991)

Hideo Yamagishi：“抗体类别转换和环状 DNA”代谢特刊“Immunology 91”28. 123-129 (1991)。

Iwasato, T., Arakawa, H., Shimizu, A., Honjo, T.and Yamagishi, H.: "Biased distribution of recombination sites within S regions upon immunoglobulin class switch recombination induced by transforming growth factor beta and lipopolysaccharide." J.Exp.Med.17

Iwasato, T.、Arakawa, H.、Shimizu, A.、Honjo, T. 和 Yamagishi, H.：“转化生长因子 β 和脂多糖诱导免疫球蛋白类别转换重组后，S 区内重组位点的偏向分布。”

山岸 秀夫: "抗体のクラススイツチと環状DNA" 代謝 増刊号「免疫'91」. 28. 123-129 (1991)

Hideo Yamagishi：“抗体类别转换和环状 DNA”代谢特刊“Immunology 91”28. 123-129 (1991)。

Hiroshi Arakawa: "The complete murine class switch α region sequence-Hierarchic structure defined by a primordial pntamer CTGRG" The Journal of Biological Chemistry. 268. 4651-4655 (1993)

Hiroshi Arakawa：“完整的鼠类开关 α 区域序列 - 由原始 pntamer CTGRG 定义的层次结构”《生物化学杂志》268. 4651-4655 (1993)。

Hiroshi Arakawa: "The complete murine class switch α region sequence-Hierarchic structure defined by a primordial pentamer CTGRG" The Journal of Biological Chemistry. 268. 4651-4655 (1993)

Hiroshi Arakawa：“完整的鼠类开关 α 区域序列 - 由原始五聚体 CTGRG 定义的层次结构”《生物化学杂志》268. 4651-4655 (1993)。