权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Intracellular signal transduction and functional regulation along the nephron segmnents.

沿肾单位段的细胞内信号转导和功能调节。

基本信息

批准号：
03454246
负责人：
ABE Keishi
金额：
$ 3.97万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

1. Cytochrome P450 represents the third metabolic pathway of arachidonic acid giving rise to several biologically active compounds, such as 19 HETE, 20 HETE and EETs and their corresponding DHETs. The kidney is the rich source of these metabolites and these metabolites have a wide and contrasting spectrum of biological and renal effects, from vasodilation to vasoconstriction and from inhibition to stimulation of Na-K-ATPase, . Their relative production rates may influence not only renal hemodynamics but also pro- and anti-hypertensive mechanisms of hypertension.2. The effect of insulin on intracellular pH reguration in microperfused rabbit S2 proximal straight tubules were examined. According to our results, insulin stimulates luminal Na-H exchange.3. The cDNA encodes 365 amino acids with 97% sequence identify to mouse EP3a receptor (EP3alpha) were isolated. Specific binding of [^3H]PGE_2 was found in COS-7 cells transfected with the cDNA (Kd=3.2nM) and was displaced with unlabeled pro … More staglandins in the order of PGE_2=PGE_1>>PGF_<2alpha>>PGD_2. In situ hybridization revealed expression of rEP_3 receptor principally in the medulla. Moreover, the reverse transcription and polymerase chain reaction (RT-PCR) using dissected nephron segments showed the receptor expression specifically in medullary and cortical thick ascending limbs of Henle's loop, cortical collecting ducts and inner medullary collecting ducts. Moreover, another isoform of EP_3 subtype (EP_<3beta>) with different carboxyl-terminal domains have been characterized.4. A cDNA encoding MDCK-type chloride channel (RKCL) was isolated. Electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. RKCL mRNA is widely expressed in various tissues. In situ hybridization revealed that RKCL mRNA is more expressed in the renal medulla and papilla than in the cortex. RT-PCR showed that main sites of the RKCL mRNA expression are ascending thin limbs of Henle's loop and inner medullary collecting ducts. Under dehydration, renal RKCL mRNA expression level was not statistically changed up to five days. Less

1.细胞色素P450代表了花生四烯酸的第三条代谢途径，产生几种生物活性化合物，如19 HETE、20 HETE和雌二醇及其相应的脱氢酶。肾脏是这些代谢物的丰富来源，这些代谢物具有广泛的生物学和肾脏效应谱，从血管舒张到血管收缩，从抑制到刺激Na-K-ATP酶。它们的相对产生率不仅影响肾脏血流动力学，而且影响高血压的促血压和降压机制.本文观察了胰岛素对微灌注兔S2近端直小管细胞内pH值的影响。根据我们的结果，胰岛素刺激管腔Na-H交换.该cDNA编码365个氨基酸，与小鼠EP 3a受体（EP 3alpha）的序列同源性为97%。在转染该cDNA的COS-7细胞中发现[^3H]PGE_2的特异性结合（Kd=3.2nM）， ...更多信息 PGE_2=PGE_1>>PGF_<2alpha>2>PGD_2。原位杂交显示rEP_3受体主要表达于髓质。此外，逆转录和聚合酶链反应（RT-PCR）使用解剖的肾单位片段显示受体的表达，特别是在髓和皮质厚的上升肢的汉勒袢，皮质集合管和内髓集合管。此外，还发现了另一种<3beta>具有不同羧基端结构域的EP_3亚型（EP_）.分离了编码MDCK型氯离子通道（RKCL）的cDNA。利用爪蟾卵母细胞表达系统进行的电生理分析显示了氯通道的功能特征。RKCL mRNA在多种组织中广泛表达。原位杂交结果显示，RKCL mRNA在肾髓质和肾乳头的表达高于皮质。RT-PCR结果显示RKCL mRNA主要表达于髓袢升支和髓内集合管。在脱水条件下，肾脏RKCL mRNA表达水平在5天内没有统计学变化。少