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Basic and clinical study of adjuvant therapy for preventaion of liver metastasis of pancreatic cancer after surgical therapy

辅助治疗预防胰腺癌术后肝转移的基础与临床研究

基本信息

批准号：
03454313
负责人：
FUKAO Katashi
金额：
$ 3.71万
依托单位：
Institute of clinical medicine, Tsukuba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

Liver metastasis of pancreatic cancer after pancreas resection is the most critical problem even now. Effective adjuvant therapy combined with surgical therapy is required for increase the survival length of the patients after pancreas resection. The prupose of this study was investigation of four ways to develop the adjuvant therapy. 1 : Arterial embolization method using fibrin glue to prevent pressing cancer cell into portal vein by surgical manipulation. 2 : Histopathological study of adjuvant irradiation to pancreas and clinical evaluation of adjuvant irradiation. 3 : Analysis of E-Cadherin DNA ・ mRNA of pancreas cancer cell which participates in invasion and metastasis. 4 : New liver function test which is useful for prediction of liver injury by ischemy during surgery or by anticancer drug infusion into the hepatic artery.Results : 1 : We determined conditions of fibrinogen and thrombin which could be injeted into small vessels. And we confirmed the fibrin glue without aprotinin in dog supra mesentric artery lysed in several hours because of plasmin like substance comtaminated in fibrin. The fibrin glue should be safe and effective in clinical use. 2 : Intra operative and post operative radiation therapy was not effective to inhibit liver metastasis after surgery but could slightly increase patient survival length. Study of pre-operative radiation method using japanese monkey is still on the way. Clinical traial of the pre-operative irradiation was started, however it takes several years to estimate the effectiveness. 3 : Four of 19 human pancreatic cancer cell lines expressed no E-Cadherin mRNA and one showed markedly reduced expression. This may explaine a molecular mechanism of invasive tendency of pancreatic cancer. 4 : We developed a new liver function test using trimethadione and have examined another new test using caffein.

胰腺癌术后肝转移是目前最严重的问题。有效的辅助治疗结合手术治疗是提高胰腺切除术后患者生存期的关键。本研究的目的是探讨四种发展辅助治疗的途径。1：使用纤维蛋白胶的动脉栓塞方法，以防止手术操作将癌细胞压入门静脉。2.胰腺辅助照射的组织学研究及临床评价。3.胰腺癌细胞E-Cadherin DNA · mRNA的表达分析第四章：新的肝功能检测方法，可用于预测术中缺血或肝动脉灌注抗癌药物引起的肝损伤。结果：1、确定了纤维蛋白原和凝血酶可注入小血管的条件。我们证实，不含抑肽酶的纤维蛋白胶在犬肠系膜上动脉中溶解数小时，因为纤维蛋白中有纤溶酶样物质污染。纤维蛋白胶在临床使用中应安全有效。第二章：术中和术后放射治疗不能有效抑制术后肝转移，但可以略微增加患者的生存时间。利用日本猴进行术前放射治疗的研究仍在进行中。开始了术前放疗的临床试验，但需要几年时间来评估疗效。3：19个人胰腺癌细胞系中有4个不表达E-Cadherin mRNA，1个表达明显降低。这可能解释了胰腺癌侵袭倾向的分子机制。4：我们开发了一种使用三甲双酮的新肝功能测试，并检查了另一种使用咖啡因的新测试。