Evaluation of two color flow cytometric analysis of peripheral immunocompetent cell phenotypes as an immunomonitoring for kidney allografted patients

外周免疫活性细胞表型的双色流式细胞术分析作为同种异体肾移植患者免疫监测的评估

• 批准号: 60570577
• 负责人: FUKAO Katashi
• 金额: $ 1.02万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570577/
• 关键词: monoclonal antibody; flowcytometry; peripheral immunocompetent cell; kidney allo-transplantation; 二重染色法

Reliable immunological monitoring method useful for the management of kidney transplanted patients has been required. Flowcytometric analysis of peripheral immunocompetent cell phenotypes using monoclonal antibody had been expected to be an ideal monitoring method.In this study, we evaluated the single color and two color flowcytometric analysis of peripheral mononuclear cell phenotypes in the management of tranplant patients. Peripheral nucleated cells of 41 kidney transplanted patients, 34 dialyzed chronic renal failure patients, 41 healty adults were examined. Cell phenotypes were analyzed using Leu series monoclonal antibodies conjugated FITC of PE and flowcytomter(FACS IV). In two color assay, we quantitated activated pan T (Leu4+DR+), activated pan T (Leu4+I12+), activated T suppressor/killer(Leu2a+DR+), T suppressor(Leu2a+15+), T killer(Leu2a+1-), T suppressor inducer(Leu3a+8+), T helper(Leu3a+8-), activated monocyte(LeuDR+M3+).Activated T suppressor/killer ratio in total cells … More of transplanted patients were higher than normal control 12 month after transplantation. T suppressor ratio of transplanted patients without acute rejection were higher than those with it, especially in the period between 3 to 12 months after surgery.T suppressor inducer 12 months after transplantation was lower and activated monocytes of transplanted patients was higher than normal control and chronic renal failure patients. Kinds of immunosuppressant did not paticular affect on the subsets ratio. Time series analysis could not find any common correlation between the alteration of cell subsets and laboratory data or clinical course. At present, we concluded that the quantitation of peripheral immunocompetent cell subsets were not so useful in management of transplanted patients. We developed new assay of phagocytic activity of macrophage using monoclonal antibody, fluorescence latex beads and flowcytometry. Now we are studying this new assay for useful monitoring for management of transplanted patients. Less

可靠的免疫学监测方法用于肾移植患者的管理一直是必需的。应用单克隆抗体流式细胞术检测外周血免疫活性细胞表型是一种理想的监测方法，本文对流式细胞术检测外周血单个核细胞表型的单色和双色两种方法在移植患者中的应用进行了评价。本文对41例肾移植患者、34例慢性肾功能衰竭透析患者及41例健康成人外周血有核细胞进行了检测。用Leu系列单克隆抗体偶联PE的FITC和流式细胞仪（FACS IV）分析细胞表型。在双色法中，我们定量了活化的泛T细胞（Leu 4 +DR+）、活化的泛T细胞（Leu 4 +I12+）、活化的T抑制/杀伤细胞（Leu 2a +DR+）、T抑制细胞（Leu 2a +15+）、T杀伤细胞（Leu 2a +1-）、T抑制诱导细胞（Leu 3a +8+）、T辅助细胞（Leu 3a +8-）、活化的单核细胞（LeuDR+M3+）。 ...更多信息 移植后12个月，移植组患者的平均血流量明显高于正常对照组。无急性排斥反应的肾移植患者T细胞抑制率高于有急性排斥反应的肾移植患者，尤其是术后3 ~ 12个月，术后12个月T细胞抑制诱导物低于正常对照组和慢性肾功能衰竭患者，活化单核细胞高于正常对照组和慢性肾功能衰竭患者。免疫抑制剂种类对亚群比例无明显影响。时间序列分析未发现细胞亚群的改变与实验室数据或临床病程之间存在任何共同的相关性。目前，我们的结论是，外周免疫活性细胞亚群的定量在移植患者的管理中并不那么有用。本文采用单克隆抗体、荧光乳胶珠和流式细胞术建立了一种新的巨噬细胞吞噬活性测定方法。现在我们正在研究这种新的检测方法，用于移植患者的管理。少

项目成果

Katashi Fukao: "Analysis of peripheral immunocompetent cell phenotypes in kidney transplanted patients" Japanese Journal of Transplantation.

Katashi Fukao：“肾移植患者外周免疫活性细胞表型分析”日本移植杂志。

深尾立: 移植.

太刀深尾：移植。

Hideo Iwasaki: "New assay of phagocytic activity of peripheral Leu M3 positive cells" Japanese Journal of Clinical Immunology.

Hideo Iwasaki：“外周 Leu M3 阳性细胞吞噬活性的新测定”，日本临床免疫学杂志。

岩崎秀生,深尾立,湯沢賢治,大塚雅昭,林正敏,岩崎洋治: 日本臨床免疫学会会誌.

Hideo Iwasaki、Tachi Fukao、Kenji Yuzawa、Masaaki Otsuka、Masatoshi Hayashi、Yoji Iwasaki：日本临床免疫学会杂志。

Katashi Fukao: "Kidney Transplantation and Immuno-monitoring" Japanese Journal of Clinical Immunology. 9. 377-381 (1986)

Katashi Fukao：“肾移植和免疫监测”日本临床免疫学杂志。