Regulation of bone metabolism - especially by bone cell interaction

在过去三年的研究中，我们获得了以下结果：1)PGE_2、IGF、IGF-β对破骨细胞形成和激活的间接作用。(1)PGE_2间接刺激破骨细胞的形成和激活，但直接抑制破骨细胞的形成和激活。(2)IGF直接和间接刺激破骨细胞的形成和激活。(3)转化生长因子-β对破骨细胞的形成和激活几乎具有相同的作用。2)用分离的兔破骨细胞分离破骨细胞特异性基因。我们分离了3个编码破骨细胞产生的特定蛋白的基因：骨桥蛋白、Oc-II和MMP9.3。分离的骨细胞对破骨细胞形成和激活的影响以及对成熟破骨细胞形成的抑制作用。

We obtained following results about this research project for the last 3 years.1.The indirect effects of PGE_2^<1)>, IGF^<2)>, IGF-beta on osteoclast formation and activation.(1)PGE_2 indirectly stimulates osteoclast formation and activation, but directly inhibits them.(2)IGF stimulates osteoclast formation and activation directly as well as indirectly.(3)TGF-beta has almost same effects on osteoclast formation.2.Isolation of osteoclast specific gene using isolated rabbit osteoclasts^<3)4)> : We isolated 3 genes encoding specific protein produced by osteoclasts : osteopontin, Oc-II and MMP-9.3.Effects of isolated osteocytes on osteoclast formation and activation, and on inhibition of matured osteoclast formation.