Regulation of bone metabolism - especially by bone cell interaction

骨代谢的调节——尤其是通过骨细胞相互作用

• 批准号: 03454423
• 负责人: KUMEGAWA Masayoshi
• 金额: $ 4.03万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454423/
• 关键词: CYTOKINES; OSTEOCYTES; OSTEOCLAST FORMATION; OSTEOCLAST FUNCTION; TGF-β; 骨吸収; 破骨細胞; osteopontin; cathepsin-L様物質; Bone cell; 骨形成; ホルモン

We obtained following results about this research project for the last 3 years.1.The indirect effects of PGE_2^<1)>, IGF^<2)>, IGF-beta on osteoclast formation and activation.(1)PGE_2 indirectly stimulates osteoclast formation and activation, but directly inhibits them.(2)IGF stimulates osteoclast formation and activation directly as well as indirectly.(3)TGF-beta has almost same effects on osteoclast formation.2.Isolation of osteoclast specific gene using isolated rabbit osteoclasts^<3)4)> : We isolated 3 genes encoding specific protein produced by osteoclasts : osteopontin, Oc-II and MMP-9.3.Effects of isolated osteocytes on osteoclast formation and activation, and on inhibition of matured osteoclast formation.

本课题经过三年的研究，取得了以下成果：1. PGE_2、IGF、IGF-β对破骨细胞形成和活化的间接影响。（1）PGE_2间接促进破骨细胞的形成和活化，而直接抑制破骨细胞的形成和活化。（2）IGF直接或间接刺激破骨细胞的形成和活化。（3）TGF-β对破骨细胞的形成有类似的作用。2.利用兔破骨细胞分离破骨细胞特异性基因的分离^<3）4）>：我们分离了3个编码破骨细胞特异性蛋白的基因：骨桥蛋白、OC-Ⅱ和MMP-9。

项目成果

Masayoshi Kumegawa: "Prostaglandin E2 Indirectly stimulates via Stromal cells, but directly inhibits, osteoclastic cell formation" Meikai Univ.Sch.Dent.22-1. 56 (1993)

Masayoshi Kumekawa：“前列腺素 E2 通过基质细胞间接刺激，但直接抑制破骨细胞形成”Meikai Univ.Sch.Dent.22-1。

Masayxoshi Kumegawa: "Prostaglandin F2 a stimulates proliferation of clonal osteoblastic MC3T3-El cells by up-regulation of insulin-like growth fatctor-I receptrs." The Joumal of Biological Chemistry. 266. 21044-21050 (1991)

Masayxoshi Kumekawa：“前列腺素 F2 a 通过上调胰岛素样生长因子-I 受体来刺激克隆性成骨细胞 MC3T3-El 细胞的增殖。”

Masayoshi Kumegawa: "Insulin-like growth factor-I supports formation and activation of osteoclasts." Endocrinology. 131. 1075-1080 (1992)

Masayoshi Kumekawa：“胰岛素样生长因子-I 支持破骨细胞的形成和激活。”

Masayoshi Kumegawa: "Prostaglandin E2 indirectly stimulates via stromal cells,but directly inhibits,osteoclastic cell formation." J.Meikai Univ.Sch.Dent.22. 56-66 (1993)

Masayoshi Kumekawa：“前列腺素 E2 通过基质细胞间接刺激，但直接抑制破骨细胞的形成。”

Tezuka,K.,et.al.: "Identification of osteopontin in isolated rabbit osteoclasts." Biochemical and Biophysical Research Communications. 186. 911-917 (1992)

Tezuka,K.,et.al.：“分离的兔破骨细胞中骨桥蛋白的鉴定”。