The effects of local factors on bone remodeling

局部因素对骨重建的影响

• 批准号: 62480371
• 负责人: KUMEGAWA Masayoshi
• 金额: $ 4.16万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480371/
• 关键词: Osteoblasts; Osteoclasts; 局所因子; 骨茅細胞

We established a new culture system for osteoclast formation from 5-fluorouracil-treated mouse spleen cells unsupported by osteoblastic cells (Kurihara et al. Blood in press). Using this system we examined the effects of factors or osteoblastic cells on osteoblastic cell formation. The results obtained were as follows. Vitamin D_3, parathyroid hormone, glucorticoids. Prostaglandin (PG) F_<2 >, IL-1( ) and SGF stimulated osteoclast formation, whereas TGF- , PGE_1 and PGE_2 inhibited the formation (Sumitani et al. Endocrinology in preparation). Osteoblastic cells were involved in osteoclast formation: undifferentiated osteoblastic cells inhibited the formation. While differentiated ones stimulated it (Hiura et al. Endocrinology in prerparation). Osteoblastic celis mediated the action of TGF- and vitamin D_3 to the precursors for osteoclasts. IL-1( ) acted also MC3T3-El cells (Ikeda et al. Calcif. Tissue Int.).

我们建立了一种新的培养系统，用于从 5-氟尿嘧啶处理的小鼠脾细胞形成破骨细胞，无需成骨细胞支持（Kurihara 等人，Blood in press）。使用该系统，我们检查了因子或成骨细胞对成骨细胞形成的影响。得到的结果如下。维生素D_3、甲状旁腺激素、糖皮质激素。前列腺素(PG)F_2、IL-1()和SGF刺激破骨细胞形成，而TGF-β、PGE_1和PGE_2抑制形成(Sumitani等人，内分泌学准备中)。成骨细胞参与破骨细胞的形成：未分化的成骨细胞抑制破骨细胞的形成。而分化的则刺激它（Hiura等人，内分泌学准备中）。成骨细胞介导 TGF-β 和维生素 D_3 对破骨细胞前体的作用。 IL-1()也对MC3T3-E1细胞起作用(Ikeda等人Calcif.Tissue Int.)。

项目成果

Kenji,Hiura; Koji,Sumitani; Terushige,Kawata; Kiyoshi,Higashino; Eiko,Kurihara; Takuya,Sato; Yoshiyuki,Hakeda; Masayoahi,Kumegawa: "Role of Mouse Osteoblastic Cells (MC3T3-E1) on Osteoblastic Cell Formation" Endocrinology.

健二、日浦；

N.Kurihara,;T.Suda,;Y.Miura,H.;Kodama,;M.Kumegawa,: Bloos in press.

N.Kurihara,;T.Suda,;Y.Miura,H.;Kodama,;M.Kumekawa,：布卢斯正在出版。

Y.Hakeda;T.Hotta;N.Kurihara;E.lkeda;N.Maeda;Y.Yagyu.M.Kumegawa: Endocrinology. 121. 1966-1974 (1987)

Y.Hakeda；T.Hotta；N.Kurihara；E.lkeda；N.Maeda；Y.Yagyu.M.Kumekawa：内分泌学。

Eiko,Ikeda; Masami,Kusaka; Yoshiyuki,Hakeda; Kazushige,Yokota; Masayoshi,Kumegawa; Shozo,Yamamoto: "Effects of Interleukin 1 Beta on Osteoblastic MC3T3-E1 Cells" Calcif. Tissue int.43. 162-166 (1988)

池田英子；

Seiichi.Ishizuka;Noriyoshi.Kurihata;Shigeyasu.Hakeda;Norihiko.Maeda;Katshumi.Ikeda;Masayoshi.Kumegawa;Anthony W.Norman: Endocrinology.

Seiichi.Ishizuka；Noriyoshi.Kurihata；Shigeyasu.Hakeda；Norihiko.Maeda；Katsumi.Ikeda；Masayoshi.Kumekawa；Anthony W.Norman：内分泌学。