The effects of local factors on bone remodeling

我们建立了一个新的培养系统，用于破骨细胞的形成，该系统由5-氟尿嘧啶处理的小鼠脾细胞不支持成骨细胞（Kurihara et al. Blood in press）。利用该系统，我们研究了成骨细胞因子或成骨细胞对成骨细胞形成的影响。获得的结果如下。维生素D_3、甲状旁腺素、糖皮质激素。前列腺素（PG）F_2<2 >、IL-1和SGF刺激破骨细胞的形成，而TGF-β、PGE_1和PGE_2抑制破骨细胞的形成（Sumitani等，Endocrinology in preparation）。成骨细胞参与破骨细胞的形成，未分化的成骨细胞抑制破骨细胞的形成。而分化的细胞则刺激了它（Hiura等，Endocrinology in preparation）。成骨细胞介导TGF-β和维生素D_3对破骨细胞前体的作用。IL-1（）也作用于MC 3 T3-E1细胞（Ikeda等人，Calcif. Tissue Int.）。

We established a new culture system for osteoclast formation from 5-fluorouracil-treated mouse spleen cells unsupported by osteoblastic cells (Kurihara et al. Blood in press). Using this system we examined the effects of factors or osteoblastic cells on osteoblastic cell formation. The results obtained were as follows. Vitamin D_3, parathyroid hormone, glucorticoids. Prostaglandin (PG) F_<2 >, IL-1( ) and SGF stimulated osteoclast formation, whereas TGF- , PGE_1 and PGE_2 inhibited the formation (Sumitani et al. Endocrinology in preparation). Osteoblastic cells were involved in osteoclast formation: undifferentiated osteoblastic cells inhibited the formation. While differentiated ones stimulated it (Hiura et al. Endocrinology in prerparation). Osteoblastic celis mediated the action of TGF- and vitamin D_3 to the precursors for osteoclasts. IL-1( ) acted also MC3T3-El cells (Ikeda et al. Calcif. Tissue Int.).