Preperation of anti HIV-1 core protein monoclonal antibody and applications for diagnosis

针对合成的氨基肉豆蔻基(N-Myr-)甘氨酸提出了新的抗体，该甘氨酸是所有N-肉豆蔻酰化蛋白质的特征。抗N-Myr-Gly-血蓝蛋白抗体可与N-Myr-Gly蛋白的N-Myr-Gly部分发生特异性反应。免疫反应可被N-Myr-Gly偶联的血蓝蛋白和白蛋白竞争，而未衍生化的蛋白质或除肉豆蔻酸以外的各种脂肪酸均不起作用。该抗体与HIV-1感染细胞裂解物的N-肉豆蔻酰化p17；因此，该抗体是研究HIV-1病毒生命周期的一种非常有用的工具。

Novel antibodies were raised against a synthetic NH_2-terminal myristoyl (N-Myr-) glycine that is characteristic of all N-myristoylated proteins. Antibody raised against N-Myr-Gly-hemocyanin reacted specifically with N-Myr-Gly moiety of N-Myr-Gly-protein. The immunoreaction was competed by hemocyanin as well as albumin conjugated with N-Myr-Gly, while underivatized proteins or various fatty acids except myristic acid had no effect. The antibody specifically reacted with the N-myristoylated p17^<gag> and precursor 55^<gag> of the HIV-1 infected cell lysate. Therefore, the antibody is shown to be very useful tool for resolution of HIV-1 virus life cycle.