Preperation of anti HIV-1 core protein monoclonal antibody and applications for diagnosis

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Novel antibodies were raised against a synthetic NH_2-terminal myristoyl (N-Myr-) glycine that is characteristic of all N-myristoylated proteins. Antibody raised against N-Myr-Gly-hemocyanin reacted specifically with N-Myr-Gly moiety of N-Myr-Gly-protein. The immunoreaction was competed by hemocyanin as well as albumin conjugated with N-Myr-Gly, while underivatized proteins or various fatty acids except myristic acid had no effect. The antibody specifically reacted with the N-myristoylated p17^<gag> and precursor 55^<gag> of the HIV-1 infected cell lysate. Therefore, the antibody is shown to be very useful tool for resolution of HIV-1 virus life cycle.