Studies for mechanisms and treatments of deep seated mycosis. --An establishment of animal models of recurrent pulmonary aspergillosis and analysis of its' pathogenicity --

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1) An establishment and analysis of recurrent model of pulmonary aspergillosisWe have established a new rat model of recurrent pulmonary aspergillus, induced by cyclophosphamide in stead of cortisone acetate. In this new model, rapid and uniform recurrence of the disease was achieved, and histopathological findings were correlate well with viable aspergillus counts of the lung at various points of the experiments.The clinical course of the recurrence was also easily estimated by the detection of aspergillus antigens in sera and urines.2) Clinical evaluation of the usefulness of antigen detection in human cases.We have recognized the usefulness of antigen detection for early diagnosis of aspergillus infection in the above model. Then serum and urine samples obtained from human clinical cases who were supposed or proven to have aspergillus infection. And we can conclude that antigen detection is useful to diagnose of aspergillosis also in human cases.3) Acute and recurrent rat models of pulmonary aspergillosis were also used for in vivo evaluations of new antifungal agents or new approaches of treatment, such as cytokines and MDPs. In these studies, we found anti-aspergillus activity of ulinastatine with combination of antifungal agents.