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Studies for mechanisms and treatments of deep seated mycosis. --An establishment of animal models of recurrent pulmonary aspergillosis and analysis of its' pathogenicity --

深部真菌病的机制和治疗研究。

基本信息

批准号：
03454569
负责人：
SOEJIMA Rinzo
金额：
$ 0.9万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

1) An establishment and analysis of recurrent model of pulmonary aspergillosisWe have established a new rat model of recurrent pulmonary aspergillus, induced by cyclophosphamide in stead of cortisone acetate. In this new model, rapid and uniform recurrence of the disease was achieved, and histopathological findings were correlate well with viable aspergillus counts of the lung at various points of the experiments.The clinical course of the recurrence was also easily estimated by the detection of aspergillus antigens in sera and urines.2) Clinical evaluation of the usefulness of antigen detection in human cases.We have recognized the usefulness of antigen detection for early diagnosis of aspergillus infection in the above model. Then serum and urine samples obtained from human clinical cases who were supposed or proven to have aspergillus infection. And we can conclude that antigen detection is useful to diagnose of aspergillosis also in human cases.3) Acute and recurrent rat models of pulmonary aspergillosis were also used for in vivo evaluations of new antifungal agents or new approaches of treatment, such as cytokines and MDPs. In these studies, we found anti-aspergillus activity of ulinastatine with combination of antifungal agents.

1)本实验采用环磷酰胺（cyclophosphamide）代替醋酸可的松（cortisone acetate）诱导大鼠肺曲霉菌病复发模型，并对其进行了动物实验研究。在这种新模型中，实现了疾病的快速和均匀复发，组织病理学检查结果与实验中不同时间点的肺曲霉菌活菌计数有很好的相关性。通过检测血清和尿液中的曲霉菌抗原也很容易估计复发的临床过程。2）抗原检测在人类病例中有用性的临床评价：我们已经认识到抗原检测在上述模型中对曲霉菌感染早期诊断的有用性。然后从临床疑似或确诊为曲霉菌感染的人中采集血清和尿液样本。我们可以得出结论，抗原检测是有用的诊断曲霉菌病也在人类的情况下。3）急性和复发性肺曲霉菌病的大鼠模型也用于体内评价新的抗真菌药物或新的治疗方法，如细胞因子和MDPs。在这些研究中，我们发现乌司他汀与抗真菌药物联合使用具有抗曲霉菌活性。