Chemical studies of floral bud forming substances

短日处理（花诱导）和长日处理的烟草植株的叶片渗出液均得到。前者表现出刺激花芽形成的活性，而后者则没有。采用连续柱层析法对短日处理烟叶渗出液中的活性物质进行了纯化。最后，得到了在0.0002 ppm时具有刺激活性的少量活性组分。所得活性物质为水溶性，具有基本性质，透析实验技术所得活性物质分子量小于1000。切割后立即用edta2na处理小叶面，收集的渗出液中含有另一种分子量更高的活性组分。

The leaf exudate was obtained from short day-treated (flower-induced) and long day-treated tobacco plants. The former short day-treated exudate exhibited flower bud formation-stimulating activity but the latter did not. The active substance contained in the exudate from short day-treated tobacco leaves was purified through successive column chromatographies. Finally, the minor amout but very active fraction exhibiting stimulating activity at 0.0002 ppm has been obtained. The active substance thus purified is water-soluble, showing a basic property, and the molecular weight must be under 1000 from dialysis experiment technique. The cut surface of the petiolets was treated with EDTA 2Na immediately after cutting, and, the exudates then collected contained another active fraction with much higher molecular weight.