Involvement of enterochromaffin (EC) cells in diarrhea

肠嗜铬 (EC) 细胞参与腹泻

• 批准号: 04454124
• 负责人: FUJITA Tsuneo
• 金额: $ 4.61万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454124/
• 关键词: Enterochromaffin cell; Serotonin; VIP; Diarrhea; Paracrine secretion

1.Serotonin-producing EC cells and VIP-containing nerves are distributed extensively in the digestive tract and are believed to be most importantly involved in the regulation of intestinal secretion. Double immunostaining demonstrated intimate association of the cells and the nerves.2.Physiological expe ; riments in vivo using canine duodenal loops support the view that diarrhea is mediated by serotonin released from EC cells, which causes liveration of VIP from vicinal nerves ; both messengers working in series as well as in parallel, potentiating the effect of each other.3.PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide) was demonstrated to intensely activate ion transport in the intestinal epithelium. Enhancement by PACAP by C1^- release was shown to be an indirect effect via stimulation of cholinergic and non-cholinergic nerves.4.Experiments using specific antagonists indicated 5-HT_3 and 5-HT_4 as the types of serotonin receptors involved in intestinal secretion.5.SEA (Staphylococeal Enterotoxin A), previously know as causeing severe vomit, was demonstrated to cause a marked enhancement of intestinal secretion (diarrhea) in dogs ; involvement of serotonin released from EC cells was suggested by electron microscope examination of the cells.

1.产生5-羟色胺的EC细胞和含VIP的神经广泛分布在消化道中，并且被认为最重要地参与肠分泌的调节。2.在体犬十二指肠袢的生理实验表明，腹泻是由EC细胞释放的5-羟色胺引起的，它引起邻近神经的VIP肝化;这两种信使既串联又平行地工作，3.腺苷酸环化酶激活多肽（PACAP）能强烈激活肠上皮细胞的离子转运。PACAP通过刺激胆碱能神经和非胆碱能神经而间接增强C1^-释放。4.使用特异性拮抗剂的实验表明，5-HT_3和5-HT_4是参与肠分泌的5-羟色胺受体类型。（葡萄球菌肠毒素A），以前被认为是导致严重呕吐，证明引起狗肠分泌的显著增强（腹泻）;通过细胞的电子显微镜检查表明涉及从EC细胞释放的5-羟色胺。

项目成果

岩永敏彦: "消化管はどのようにして食物を認識するか" 化学と生物. 31. 650-658 (1993)

Toshihiko Iwanaga：“消化道如何识别食物”化学与生物学 31. 650-658 (1993)。

Iwanaga,Toshihiko: "Enterochromaffin (EC) cells in the gut with special reference to their role in diarrhea." Biomed.Res.(Suppl.). (1993)

Iwanaga,Toshihiko：“肠道中的肠嗜铬 (EC) 细胞，特别是它们在腹泻中的作用。”

Kuwahara A.: "5-hydroxytryptamine (5-HT)-induced anion secretion in isolated guinea-pig distal colon." J.Physiol.446. 197 (1992)

Kuwahara A.：“5-羟色胺 (5-HT) 诱导离体豚鼠远端结肠中的阴离子分泌。”

Iwanaga T.: "Functional significance of serotonin in the brain, gut and urethra : A multipotential brain-gut hormone regulating activity of neurons" J.Brain-Gut-Hormones. 8. 1-9 (1993)

Iwanaga T.：“血清素在大脑、肠道和尿道中的功能意义：调节神经元活动的多能脑肠激素”J.Brain-Gut-Hormones。

Iwanaga T.: "The topographical relation between serotonin-containing paraneurons and peptidergic neurons in the intestine and urethra" Biol.Signals. IN press. (1994)

Iwanaga T.：“肠道和尿道中含血清素旁神经元与肽能神经元之间的拓扑关系”Biol.Signals。