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Localization and functional significance of signal substances in the urogenital tract

泌尿生殖道信号物质的定位和功能意义

基本信息

批准号：
04304027
负责人：
FUJITA Tsuneo
金额：
$ 8.96万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304027/
关键词：
Corpus cavernosum penis Erection Urethra VIP Serotonin PACAP

项目摘要

1. Nerves supplying the corpus cavernosum penis were dissected and observed with a binocular microscope and their precise course and distribution were demonstrated. This information will contribute for preserving erectile nerves in surgery.2. VIP, one of the transmitters involved in erection, was examined by immunohistochemistry and by in situ hybridization with regard to its occurrence in penile nerves and to the changes in cases of impotence.3. Nerves producing NO, another signal substance causing erection, were identified, by immunohistochemical detection of NO-synthase, in pelvic ganglia which extended immunoreactive fibers to the corpus cavernosum. NO-synthase was recognized also in sinus endothelium, suggesting a correlation between neurons and endothelial cells in penile erection.4. Experiments in vitro using tissue pieces of cavernous body indicated that smooth muscles of the cavernous body relaxd by VIP and acetylcholine, this effet being demonstrated to be testosterone-dependent, while contracted by adrenalin, expressing alpha1 adrenalin receptor.5. Radioimmunoassay of human reproductive organs indicated that the testis contained not so much VIP as PACAP and that the seminal vesicles were characterized by high content of NPY.6. The urinary tract was shown by immunohistochemistry for substance P and CGRP to be densely supplied by sensory nerves. They are distributed mainly to smooth muscles in the pelvis and ureter, while into the epithelium in the urethra. The essential role of these nerves is sensory but presumably they are involved in regulation of peristaltic movement in the former organs and in provoking sexual sensation in the latter.

1.在双目显微镜下解剖观察了阴茎海绵体的神经，明确了其走行和分布。这些信息将有助于在手术中保留勃起神经。采用免疫组织化学和原位杂交方法，观察了与勃起有关的递质之一VIP在阴茎神经中的分布及在阳痿患者中的变化.产生NO的神经，另一种信号物质引起勃起，确定，通过免疫组化检测NO合酶，在盆神经节的免疫反应纤维延伸到阴茎海绵体。NO合酶在窦内皮也有表达，提示阴茎勃起过程中神经元和内皮细胞之间存在相关性.离体海绵体组织块实验表明，VIP和乙酰胆碱可使海绵体平滑肌松弛，这种作用是睾酮依赖性的，而肾上腺素可使海绵体平滑肌收缩，表达α 1肾上腺素受体.人生殖器官放射免疫分析表明，睾丸中VIP含量低于PACAP，精囊中NPY含量较高. P物质和CGRP的免疫组织化学显示，尿路密集的感觉神经供应。它们主要分布于肾盂和输尿管的平滑肌，而尿道的上皮中。这些神经的基本作用是感觉，但它们可能参与调节前一器官的蠕动运动，并引起后一器官的性感觉。