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Morphological analysis of liver regeneration and the effects of gastro-entero-pancreatic hormones upon it.

肝再生的形态学分析及胃肠胰激素对其的影响。

基本信息

批准号：
62440021
负责人：
FUJITA Tsuneo
金额：
$ 18.94万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

This study aims at the detection of trophic effects of GEP (gastro-entero-pancreat-ic) hormones upon the liver after partial hepatectomy. The effects upon the pancreas were also examined.1. In the mouse repeated and combined injections of glucagon (150 mug/kg/day) and insulin (1.5 U/kg/day) caused 50% increase in liver weigth. In the rat the glucagon-insulin treatment was effective when oral administration of trypsin inhibitor (TI)(1.5 mg/ml) was combined, leading to 20% weight increase. The normal (non-hepatectomized) liver did not react to those treatments. Neither secretin (140 U/kg/day) nor CCK8 (10 mug/kg/day) exerted detectable trophic effect upon the regenerating liver.2. In order to determine wheter the trophic effects of the hormones might be due to proliferation of liver cells or due to their hypertrophy, the hepatic tissue after the glucagon-insulin-TI (or CCK) treatment was immunohistochemically examined after labelling mitotic cells with BrdU. Essentially no difference could be found between the treated and non-treated cases in the frequency and distribution of labelled cells. Hypertrophy thus seems to be the main, if not exclusive, process of the trophic effect.3. The pancreas increased in weight after hepatectomy, for unknown reasons. This effect was enhanced in the animals treated with glucagon-insulin, secretin, CCK8 or TI.4. Morphological studies indicated that the regenerating liver showed acinar construction and biliary arborization similar to the states in newborns and lower vertebrates.

本研究旨在检测部分肝切除术后 GEP（胃肠胰）激素对肝脏的营养作用。还检查了对胰腺的影响。1．在小鼠中，重复联合注射胰高血糖素（150 微克/公斤/天）和胰岛素（1.5 U/公斤/天）导致肝脏重量增加 50%。在大鼠中，当联合口服胰蛋白酶抑制剂 (TI) (1.5 mg/ml) 时，胰高血糖素-胰岛素治疗有效，导致体重增加 20%。正常（未肝切除的）肝脏对这些治疗没有反应。促胰液素（140 U/kg/天）和CCK8（10 μg/kg/天）均未对再生肝脏产生可检测到的营养作用。2．为了确定激素的营养作用可能是由于肝细胞增殖还是由于其肥大，在用BrdU标记有丝分裂细胞后，对胰高血糖素-胰岛素-TI（或CCK）处理后的肝组织进行免疫组织化学检查。在标记细胞的频率和分布方面，处理和未处理的病例之间基本上没有发现差异。因此，肥大似乎是营养效应的主要（如果不是唯一的）过程。3．肝切除术后，胰腺重量增加，原因不明。在用胰高血糖素-胰岛素、促胰液素、CCK8或TI.4治疗的动物中，这种效果得到增强。形态学研究表明，再生的肝脏表现出与新生儿和低等脊椎动物相似的腺泡结构和胆道树枝化。