Morphological analysis of liver regeneration and the effects of gastro-entero-pancreatic hormones upon it.

本研究旨在检测GEP(胃肠胰脏激素)对肝部分切除后肝脏的营养作用。对胰腺的影响也进行了检测。在小鼠中，反复注射胰高血糖素(150 mg/kg/d)和胰岛素(1.5 U/kg/d)可使小鼠肝脏重量增加50%。当大鼠口服胰酶抑制剂(TI)(1.5 mg/ml)时，胰升糖素-胰岛素治疗有效，体重增加20%。正常(未切除肝)的肝脏对这些治疗没有反应。结论：1.促胰液素(140 U/kg/d)和CCK8(10µg/kg/d)对再生肝均无明显的营养作用。为了确定激素的营养作用可能是由于肝细胞的增殖还是由于其肥大，用BrdU标记有丝分裂细胞后，用胰高血糖素-胰岛素-TI(或CCK)处理后的肝组织进行了免疫组织化学检测。在治疗和未治疗的病例中，标记细胞的频率和分布基本上没有差异。因此，肥大似乎是营养效应的主要过程，如果不是排他性的话。肝脏切除后，胰腺重量增加，原因不明。这种作用在用胰升糖素-胰岛素、促胰液素、CCK8或TI.4治疗的动物中得到加强。形态学研究表明，再生肝具有与新生儿和低等脊椎动物相似的腺泡结构和胆管树枝状结构。

This study aims at the detection of trophic effects of GEP (gastro-entero-pancreat-ic) hormones upon the liver after partial hepatectomy. The effects upon the pancreas were also examined.1. In the mouse repeated and combined injections of glucagon (150 mug/kg/day) and insulin (1.5 U/kg/day) caused 50% increase in liver weigth. In the rat the glucagon-insulin treatment was effective when oral administration of trypsin inhibitor (TI)(1.5 mg/ml) was combined, leading to 20% weight increase. The normal (non-hepatectomized) liver did not react to those treatments. Neither secretin (140 U/kg/day) nor CCK8 (10 mug/kg/day) exerted detectable trophic effect upon the regenerating liver.2. In order to determine wheter the trophic effects of the hormones might be due to proliferation of liver cells or due to their hypertrophy, the hepatic tissue after the glucagon-insulin-TI (or CCK) treatment was immunohistochemically examined after labelling mitotic cells with BrdU. Essentially no difference could be found between the treated and non-treated cases in the frequency and distribution of labelled cells. Hypertrophy thus seems to be the main, if not exclusive, process of the trophic effect.3. The pancreas increased in weight after hepatectomy, for unknown reasons. This effect was enhanced in the animals treated with glucagon-insulin, secretin, CCK8 or TI.4. Morphological studies indicated that the regenerating liver showed acinar construction and biliary arborization similar to the states in newborns and lower vertebrates.