Molecular basis of neural plasticity in the hippocampus.

海马神经可塑性的分子基础。

• 批准号: 04454142
• 负责人: OBATA Kunihiko
• 金额: $ 4.22万
• 依托单位: NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454142/
• 关键词: Hippocampus; neuronal plasticity; kindling; kainic acid; molecular cloning; protein phosphorylation; long-term potentiation; synapsin I; チトクロム・オキシダーゼ; ミトコンドリアDNA

In order to elucidate the molecular mechanism of neuronal plasticity, we investigated specific changes in gene expression and protein phosphorylation of the rat hippocampus after kindling or long-term potentiation was induced. Molecular cloning was performed on the subtracted cDNA library prepared from kindled and control hippocampi. Expression of genes for cytochrome oxidase subunits 2 and 3 was elevated by kindling. Protein phosphorylation by calcium/calmodulin-dependent kinase II was investigated on the hippocampus and cerebral cortex after kindling. Although enzyme activity was not altered significantly, phosphorylation of synapsin I was elevated in kindled hippocampus and cortex. Glutamate release from synaptosome fraction was investigated in vitro. High potassium stimulation induced the more release from kindled cortex.

为了阐明神经元可塑性的分子机制，我们研究了点燃或长时程增强诱导后大鼠海马区基因表达和蛋白磷酸化的特异性变化。对点燃组和对照组大鼠海马区消减文库进行分子克隆。点燃后，细胞色素氧化酶亚基2和3的基因表达增加。研究了点燃后大鼠大脑皮层和海马区钙/钙调素依赖性激酶II的蛋白磷酸化作用。虽然酶活性没有明显改变，但点燃的海马区和皮质区突触素I的磷酸化水平升高。研究了突触体部分体外释放谷氨酸的情况。高钾刺激可使点燃的大脑皮层释放更多的钾。

项目成果

小幡邦彦: "海馬における神経活動と遺伝子発現" 神経研究の進歩. 38. 82-89 (1994)

Kunihiko Obata：“海马体中的神经活动和基因表达”神经学研究进展 38. 82-89 (1994)。

丸山 敬 他: "ラット海馬の長期増強(LTP)に関連して発現する遺伝子の検索の21GC06:神経化学" 33. 616-617 (1994)

Takashi Maruyama 等人：“寻找与大鼠海马 21GC06 中长时程增强 (LTP) 相关的基因表达：神经化学”33. 616-617 (1994)

Yamagata Y.et al.: "Increase in syanapsin I phosphorylation implicates a presynaptic component in septal kindling." Neuroscience. 64. 1-4 (1995)

Yamagata Y.et al.：“syanapsin I 磷酸化的增加表明间隔点燃中存在突触前成分。”

Kunihiko Obata: "Relation between learning impairment,hippocampal pyramidal cells loss and other collular cghangesin the rait after seigare induced by kainic acid and pilocarpine" Neuroscience Research. Suppl.18. 153- (1993)

Kunihiko Obata：“红藻氨酸和毛果芸香碱诱导的学习障碍、海马锥体细胞损失和其他脑细胞变化之间的关系”神经科学研究。

児島 伸彦,小幡 邦彦: "キンドリングにおける遺伝子発現の変化" 神経精神薬理. 15. 85-92 (1993)

Nobuhiko Kojima、Kunihiko Obata：“点燃过程中基因表达的变化”《神经精神药理学》15. 85-92 (1993)。