Molecular basis of neural plasticity in the hippocampus.

点击翻译按钮获取中文摘要

In order to elucidate the molecular mechanism of neuronal plasticity, we investigated specific changes in gene expression and protein phosphorylation of the rat hippocampus after kindling or long-term potentiation was induced. Molecular cloning was performed on the subtracted cDNA library prepared from kindled and control hippocampi. Expression of genes for cytochrome oxidase subunits 2 and 3 was elevated by kindling. Protein phosphorylation by calcium/calmodulin-dependent kinase II was investigated on the hippocampus and cerebral cortex after kindling. Although enzyme activity was not altered significantly, phosphorylation of synapsin I was elevated in kindled hippocampus and cortex. Glutamate release from synaptosome fraction was investigated in vitro. High potassium stimulation induced the more release from kindled cortex.