Human model system for angiogenesis and molecular mechanisms on function of growth factors involving in the angiogenesis

我们从人大网膜中分离出微血管内皮细胞并成功体外培养。(1)当融合培养的人网膜微血管（HOME）细胞被剃刀损伤时，这种细胞在EGF/ tgf - α的作用下最有效地迁移。当EGF/ tgf - α存在时，胶原凝胶中的管生成以及组织型纤溶酶原激活物（t-PA）的表达也有效增强。(2)用tnf - α处理HOME细胞可抑制HOME细胞的细胞迁移和小管形成。tnf - α可进一步诱导HOME细胞中IL-6、bFGF和胶原酶基因的表达。(3)我们也可以建立一个新的共培养体系，以确定共培养细胞是否能诱导胶原凝胶中的内皮细胞成管。在这个系统中，人角化细胞和食管癌细胞可能通过分泌tgf - α控制旁分泌来诱导HOME细胞的小管形成。在我们的实验室中，我们正在培养系统中分离与HOME细胞血管生成有关的基因。

We have isolated microvascular endothelial cells from human omentum and successfully cultured in vitro.(1) This human omentum microvascular (HOME) cells were found to be most effectively migrated in response to EGF/TGF-alpha when confluent cultured HOME cells were wounded with razor. Tubulogenesis in collagen gel as well as expression of tissue-type plasminogen activator (t-PA) was also effectively enhanced when EGF/TGF-alpha was present.(2) Treatment of HOME cells with TNF-alpha inhibited both cell migration and tubulogenesis of HOME cells. Furthermore TNF-alpha could further induce expression of IL-6, bFGF and collagenase genes in HOME cells.(3) We could also establish a new co-culture system to determine whether co-cultured cells could induce tubulogenesis of endothelial cells in collagen gels. In this system, human keratinocytes and esophageal cancer cells could induce tubulogenesis of HOME cells possibly through paracrine control by secretory TGF-alpha. In our laboratory, we are nocoisolating genes involving in angiogenesis of HOME cells in culture system.