Peripheral and central involvement of 5-hydroxytryptamine in control of interdigestive migrating contractions by motilin.

5-羟色胺的外周和中枢参与胃动素控制消化间期迁移性收缩。

• 批准号: 04454239
• 负责人: ITOH Susumu
• 金额: $ 3.84万
• 依托单位: Gunma Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454239/
• 关键词: Motilin; Interdigestive migrating complex; Serotonin

1. In order to study the role 5-hydroxytryptamine in the peripheral action of motilin, the canine stomach was perfused with artificial blood after the stomach was isolated from the donor dog. As a result, motilininduced contractions migrated toward the pyloric ring in the isolated stomach were completely inhibited by treatment of the perfused stomach with a 5-hydroxytryptamine 3 receptor antagonist. It is concluded that 5-hydroxytryptamine is involved in the action of motilin in the isolated stomach. In the same system, it was also found that alpha-adrenergic receptors and the cholinergic neuron were involved in the action of motilin.2. The effects of various receptor antagonists on EM523-induced contractions in the gastrointestinal tract were studied in conscious dogs in which force trasducers were chronically implanted to measure gastrointestinal contractions. EM523, a non-peptide motilin agonist, was found to induce phase III-like contractions in the stomach in a dose-related manner with concomitant increase in the plasma consentration of motilin. The EM523-induced contractions in the stomach were eliminated by pretreatment of dogs with cholinergic and 5-hydroxytryptamine 3 receptor antagonists, and dopamine, but the contractions were not influenced by opiate (mu) receptor antagonist and methysergide. These findings obtained in conscious dogs agree with those found in the isolated stomach.3 In the present study, it was revealed that 5-hydroxytryptamine neuron is involved in the control of contractions in the gastrointestinal tract by motilin, but the relationship between 5-hydroxytryptamine neuron and motilin receptors in the stomach has not been elucidated ; this should be clarified in the future. The role of 5-hydroxytryptamine in the CNS in the control of the interdigestive migrating contractions by motilin has been also studied, but no definite conclusion could be obtained ; further studies are needed.

1.为了研究5-羟色胺在胃动素外周作用中的作用，从供体犬分离胃后，对犬胃进行人工血液灌注。结果，用 5-羟色胺 3 受体拮抗剂处理灌注的胃完全抑制了胃动素诱导的向离体胃中幽门环迁移的收缩。结论是5-羟色胺参与胃动素在离体胃中的作用。在同一系统中，还发现α-肾上腺素能受体和胆碱能神经元参与胃动素的作用。2．在有意识的狗中研究了各种受体拮抗剂对 EM523 诱导的胃肠道收缩的影响，其中长期植入力换能器以测量胃肠道收缩。 EM523 是一种非肽胃动素激动剂，被发现能以剂量相关的方式诱导胃中的 III 期样收缩，同时增加胃动素的血浆浓度。用胆碱能和5-羟色胺3受体拮抗剂以及多巴胺对狗进行预处理可以消除EM523诱导的胃收缩，但收缩不受阿片(mu)受体拮抗剂和美西麦角的影响。在有意识的狗中获得的这些结果与在离体胃中发现的结果一致。 3 在本研究中，揭示了 5-羟色胺神经元参与胃动素对胃肠道收缩的控制，但 5-羟色胺神经元与胃中胃动素受体之间的关系尚未阐明；今后应该澄清这一点。中枢神经系统中5-羟色胺在胃动素控制消化间期游走性收缩中的作用也已被研究，但尚未得出明确的结论；还需要进一步研究。

项目成果

Mlzumoto,A.: "Mechanism of motilin-induced contractions in isolated perfused canie stomach." Gastroenterology. 105. 425-432 (1993)

Mlzumoto,A.：“胃动素诱导离体灌注犬胃收缩的机制。”

Ohtawa, M., Mizumoto, A., Hayashi, N., Yanagida, K., Itoh, Z., Omura, S.: Gastroenterology. 104. 1320-1327 (1993)

Ohtawa, M.、Mizumoto, A.、Hayashi, N.、Yanagida, K.、Itoh, Z.、Omura, S.：胃肠病学。

Inatomi N: "Mechanism of the motor-stimulating action of motility and motilide (EM574)in the dog." Regulatory Peptides. 40. 172 (1992)

Inatomi N：“狗的动力和吗替利特 (EM574) 的运动刺激作用机制。”

Inatomi, N., Satoh, T., Satoh, H., Itoh, Z., Omura, S.: "Comparison of the moter-stimulating action of EM523, an erythromycin derivative, and prostaglandin F2alpha in conscious dogs." Japan.J.Pharmacol. 63. 209-217 (1993)

Inatomi, N.、Satoh, T.、Satoh, H.、Itoh, Z.、Omura, S.：“红霉素衍生物 EM523 和前列腺素 F2α 在清醒狗中的运动刺激作用的比较。”

Mizumoto,A.: "Mechanism of motilin-induced contractions in isolated perfused canie stomach." Gastroenterology. 105. 425-432 (1993)

Mizumoto,A.：“胃动素诱导离体灌注犬胃收缩的机制。”