STUDY ON PATHOPHYSIOLOGICAL SIGNIFICANCIE OF BILIRUBIN

胆红素的病理生理意义研究

• 批准号: 13670813
• 负责人: ITOH Susumu
• 金额: $ 1.92万
• 依托单位: KAGAWA UNIVERSITY, FACULTY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670813/
• 关键词: BILIRUBIN; ANTIOXIDANT EFFECTS; PROPENTDYOPENTS; BILIVERDIN; ACTIVE OXYGEN SPECIES; NEONATAL PERIOD; BIOPYRRIN; 8-HYDROXYDEOXYGUANOSINE; プロペントディオペント; スーパオキサイドアニオン; 光療法; グリーンライト; プロベンドディオペント; 活性酵素

Background : The antioxidant effect of bilirubin in perinatal period came to be found as the pathophysiological significance of active oxygen was found. Bilirubin scavenges or quenches active oxygen species and lipid peroxides etc. Scavenged bilirubin is oxidized to propentdyopents, and quenched bilirubin is oxidized to biliverdin. However, the pathophysiological significance of these bilirubin oxidative products in neonatal period is unclear. We studied the bilirubin oxidative productsMethods In an In vitro study we measured the amounts of oxidative products of bilirubin formed through the action of O_2^-by the xanthine-xanthine oxidase system, either as free bilirubin or as bilirubin-human serum albumin complex. In an in vivo study we measured the amounts of serum biliverdin and urinary propentdyopents that were produced by phototherapy or oxygen therapy. We measured biopyrrin examined by using a Biopyrrin EIA kit and 8-hydroxydeoxyguanosine examined by using an 8-OhdG ELISA kit in neonatal urine before and after neonatal jaundiced phototherapyResults : In the in vitro study, a significant formation of propentdyopents was observed in the both reaction solution, but biliverdin was not detected. In the in vivo study, biliverdin was not reflected in generation of active oxygen species. Biopyrrin was difficult to evaluate 'the antioxidant effects of bilirubin because of bilirubin photoisomers and also 8-hydroxydeoxyguanosine was difficult to evaluate the production of active oxygen species because of the impurities in neonatal urine. Conclusion : Further study on the production of propentdyopents production from (ZZ)-bilirubin in vivo is needed

背景资料：胆红素在围产期的抗氧化作用是随着活性氧的病理生理学意义的发现而发现的，胆红素清除或淬灭活性氧和脂质过氧化物等，清除的胆红素被氧化为丙戊酸盐，淬灭的胆红素被氧化为胆绿素。然而，这些胆红素氧化产物在新生儿期的病理生理意义尚不清楚。方法在体外实验中，我们测定了胆红素在黄嘌呤-黄嘌呤氧化酶体系中与O_2^-作用时，以游离胆红素或胆红素-人血清白蛋白复合物形式生成的氧化产物的量。在一项体内研究中，我们测量了光疗或氧疗产生的血清胆绿素和尿中的ppropentdyopents的量。用BiopyrinEIA试剂盒检测新生儿黄疸光疗前后尿中的Biopyrin，8-OHDGELISA试剂盒检测新生儿黄疸光疗前后尿中的8-羟基脱氧鸟苷。在体内研究中，胆绿素没有反映在活性氧的产生。由于胆红素的光异构体，生物吡咯难以评价胆红素的抗氧化作用，并且由于新生儿尿液中的杂质，8-羟基脱氧鸟苷也难以评价活性氧的产生。结论：（ZZ）-胆红素体内合成丙戊二酸的研究尚需进一步深入

项目成果

Yasuda S, Itoh S, Imai T et al.: "Cyclobilirubin formation by in vitro photoirradiation with neonatal phototherapy light."Pediatr Int. 43. 270-275 (2001)

Yasuda S、Itoh S、Imai T 等人：“新生儿光疗光体外光照射形成环胆红素。”Pediatr Int。

Kondo M, Itoh S, Kusaka T et al.: "Time of closure of ductus venosus in term and preterm neonates"Arch Dis Child Fetal Neonatal Ed. 85. F57-F59 (2001)

Kondo M、Itoh S、Kusaka T 等：“足月和早产儿静脉导管闭合时间”Arch Dis Child Fetal Neonatal Ed。

伊藤 進: "Breast milk jaundice, breast feeding jaundice 症候群"小児科診療. 64. 430 (2001)

Susumu Ito：“母乳黄疸，母乳喂养黄疸综合征”儿科学 64. 430 (2001)。

Yasuda S, Itoh S, Imai T, et al.: "Cyclobilirubin formation by in vitro photoirradiation with neonatal phototherapy light"Pediatr Int. 43. 270-275 (2001)

Yasuda S、Itoh S、Imai T 等人：“新生儿光疗光体外光照射形成环胆红素”Pediatr Int。

Itoh S.: "Measurement of serum bilirubin and its subfractions by HPLC"Clin Chem Lab Med. 40. S40 (2002)

Itoh S.：“通过 HPLC 测量血清胆红素及其亚组分”Clin Chem Lab Med。