Reovirus type 3Infection in Biliary Atresia

呼肠孤病毒 3 型胆道闭锁感染

• 批准号: 04454279
• 负责人: MATSUI Akira
• 金额: $ 1.15万
• 依托单位: JICHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454279/
• 关键词: Bi liary atresia; Reovirus type 3

The aim of the present study was 1) to perform immunohistochemistry of livers obtained during Kasai operation for infants with biliary atresia (BA) using anti-reovirus type 3 (R3), 2) to detect the RNAs of R3 from those livers with reverse transcriptase-polymerase chain reaction and 3) to elucidate pathophysiology of biliary obstruction in the neonatal mice inoculated with R3. We summarise our results as follows ; 1) 24 of 25 livers of BA reacted with anti-R3 (Abney strain : A) polyclonal antibodies, but none with anti-R3 (Dearing strain : D) monoclonal ones probably because of these weaker staining activities. 2) M1 segment of D was detected in both 3livers of BA and 2 with non-liver disease controls. 3) The liver and choledochus of neonatal mice inoculated with R3-A developed histologically similar features with human biliary atresia such as degeneration of ductular epithelia, surrounded with mononuclear infiltration, and positive R3-A associated antigens. A recent report suggests that a part of S1 segment of R3-A may play a role in determining the tropism of this virus for ductular epithelium. Therefore we believe we should try to detect this part with reverse transcription-polymerase chain reaction. Although we could not conclude that R3 causes BA in the present study, our results strongly suggest that R3-A infection may trigger the obliterative process of sclerosing cholangitis in BA.

本研究的目的是1）使用抗呼肠孤病毒3型（R3）对患有胆道闭锁（BA）的婴儿进行加塞手术期间获得的肝脏进行免疫组织化学，2）使用逆转录酶-聚合酶链反应检测来自这些肝脏的R3的RNA，3）阐明接种R3的新生小鼠中胆道梗阻的病理生理学。我们总结了我们的结果如下：1）24/25的BA肝与抗R3（Abney株：A）的多克隆抗体反应，但没有与抗R3（Dearing株：D）的单克隆抗体反应，可能是因为这些较弱的染色活性。2)30例BA患者和2例非肝病对照者的肝脏均检测到D的M1段。3)接种R3-A的新生小鼠的肝脏和胆总管出现了与人类胆道闭锁相似的组织学特征，例如小管上皮细胞变性，周围有单核细胞浸润和阳性R3-A相关抗原。最近的报道表明，R3-A的S1片段的一部分可能在决定该病毒对导管上皮的嗜性方面起作用。因此，我们认为，我们应该尝试检测这部分的逆转录聚合酶链反应。虽然我们不能得出结论，R3导致BA在本研究中，我们的研究结果强烈表明，R3-A感染可能会触发闭塞过程中的硬化性胆管炎的BA。

项目成果

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