Rapid Diagnosis of Infectious Diseases Using PCR

在这项研究中，聚合酶链反应被用来作为一种快速和敏感的方法检测传染性病原体，其中四个检测系统的设计，包括人T细胞白血病病毒I型，结核分枝杆菌，支原体和麻疹病毒的扩增方法。在对人类T细胞白血病病毒I型的研究中，在使用来自该病毒携带者母亲的脐带血单核细胞的实验中证明了该病毒通过脐带血垂直传播的可能性。在结核分枝杆菌的研究中，该方法被证明是有效的，特别是在肺外病例中检测该微生物。在肺炎支原体研究中，获得了该病原体直接侵入中枢神经系统以及支原体中枢神经系统受累时伴随发生支原体血症的证据。4例麻疹脑炎患者中，3例在发病后1天内脑脊液中检出麻疹病毒RNA，提示麻疹病毒在急性麻疹脑炎的病程早期即从中枢神经系统中清除，证实了聚合酶链反应在传染病临床研究中的应用价值。

In this study polymerase chain reaction was used as a rapid and sensitive method for detecting infectious agents for which four assay systems were devised comprising the methods for the amplification of human T cell leukemia virus type I, Mycobacterium tuberculosis, Mycoplasma Pneumoniae and measles virus. In the study on human T cell leukemia virus type I, the possibility of vertical transmission of this virus through cord blood was demonstrated in the experiment using cord blood mononuclear cells from carrier mothers of this virus. In the study on Mycobacterium tuberculosis, this method was shown to be efficient, particularly in detecting this organism in extrapulmonary cases. In the study on Mycoplasema pneumoniae, evidence of direct invasion into the central nervous system by this orgenism and the concomitant occurrence of mycoplasmaemia in the mycoplasmal central nervous system involvement was obtained. Measles virus RNA was detected in 3 of 4 patients with measles encephalitis in the cerbrospinal fluid samples obtained within a day of the onset of neurological symptoms suggesting that the virus is cleared from the central nervous system very early in the usual course of an acute measles encephalitis.These results validated the potential of polymerase chain reaction in clinical research of infectious diseases.