Investigation if Pathogenesis of SSPE Using Molecular Biological Techniques.

在前期的研究中，应用生物素标记的DNA探针原位杂交技术成功地研究了麻疹病毒基因组在SSPE脑组织中的定位。然而，为了进一步促进这一技术的推广，必须提供更直接和客观的可用性证据。本研究以人T细胞白血病病毒Ⅰ型（HTLV-Ⅰ）为研究对象，它作为前病毒基因组DNA整合在细胞核中，检测时具有特异性，并揭示了它与艾滋病或自身免疫性疾病的关系。该病毒的经胎盘传播是由以下事实提出的：在5例病例中有2例从脐带血单核细胞扩增出HTLV-I特异性条带。第二，在HTLV-I的中枢神经系统感染中，即HTLV-I相关的脊髓病，从这些患者的微量CSF样品中扩增出非常强的信号，这一事实表明该病毒的感染处于活跃状态。如上所述，该系统有效地应用于临床研究。随着技术的不断改进，非放射性同位素原位杂交技术将在临床研究中发挥更重要的作用。

In a preceding study, a method of in situ hybridization using biotinlabelled DNA probe was successfully applied to investigate localization of measles virus genome in SSPE brain tissue. However, to facilitate further a spread of this technique, more direct and objective evidence of availability must be shown. For this purpose, polymerase chain reaction-non-radioisotopic detection system was constructed.Human T-cell leukemia virus type I (HTLV- I) was used as a target of this study, because, it was integrated in nuclei as proviral genome DNA, pathognomonic when detected, and shed on light in relation to AIDS or autoimmune diseases.First, in mother to child transmission, transplacental transmission of this virus was suggested by a fact that there existed 2 out of 5 cases in which HTLV-I specific bands were amplified from cord blood mononuclear cells.Second, in central nervous system infection of HTLV-I, that is, HTLV-I associated myelopathy, an active state of infection of this virus was suggested by a fact that remarkably strong signals were amplified from minute amount CSF samples of these patients.As mentiond above, this system was efficiently applied to clinical research. With more modifications, non-radioisotopic in situ hybridization will share a more important part of clinical investigations.