Basic research for Gd-NCT

当前的BNCT不能摧毁肿瘤细胞，因为它不会把它们拿走，因为阿尔法粒子太短了，不能用来刺激邻居细胞。为了打破这一限制，我们研究了使用Gd-DTPA的Gd-NCT的可能性.在文化SCCVII肿瘤细胞中测试了Gd-NCT的细胞杀伤效应，显示了BNCT对所有药物cncentrations的次级效应; 2、3和5毫米特别是，细胞存活曲线在2毫米肩下。在体内系统中， Gd-NCT显示最大效应在Gd-DTPA注射后2-3分钟，当细胞因子被阻断的微核试验评价时，该效应。在菌落形成分析中， Gd^<157>N-DTPA（0.6 μ mol/g小鼠身体重量）与B^<10>NPA（750毫克/g，平均B^<10>N浓度在肿瘤= 0.7 μ mol/g）中的影响相似。我们先前已将Alpha组件的细胞生存曲线完全适用于与正常核分区（NNDF）的部分; without micronucleus appearance nor uneven nuclear division。由于SCCVII肿瘤跟随Gd-NCT是线性的，B^ N分布在细胞水平上的同源性<10>可以通过比较细胞生存曲线和NNDF曲线进行调查。NNDF曲线显示出与细胞存活曲线跟随Gd-NCT的精确协议;-lnNNDF =0.137·T（m）对- lnSF=0.131·T（m）。在BPA-NCT中， However，两条曲线显示出一种分心。这些发现意味着Gd-DTPA比BPA更均匀。

The current BNCT can not destroy the tumor cells which don't take up boron because alpha particle is too short for strilizing neighbor cells. To break this limitation, we studied the possibility of Gd-NCT using Gd-DTPA.The cell killing effect of Gd-NCT examined in cultured SCCVII tumor cells presented the inferior effect to that of BNCT at all drug cncentrations ; 2,3 and 5 mM.Especially, the cell survival curve at 2 mM has shoulder. In vivo system, Gd-NCT showed maximum effect 2-3 min after the injection of Gd-DTPA, when the effect was asessed by cytokinesis blocked micronucleus assay. In colony formation assay, Gd^<157>N-DTPA(0.6 mu mole/g mouse body weight) yielded the effect being similar to that of B^<10>NPA(750 mg/g, average B^<10>N concentration in tumors = 0.7mu mole/g). We previously elucidated that alpha component of cell survival curve completely appreared as the fraction of cells with normal nuclear division (NNDF) ; without micronucleus appearance nor uneven nuclear division. Since the cell survival curve of SCCVII tumors following Gd-NCT is linear, the homogeneity of B^<10>N distribution at cellular level can be investigated by comparing cell survival curve and NNDF curve. The NNDF curve showed precise agreement with the cell survival curve following Gd-NCT ; -lnNNDF=0.137・T(m) vs.-lnSF=0.131・T(m). In BPA-NCT, however, two curves showed a discrepancy. These findings mean that Gd-DTPA distributed more homogeneously than BPA.