Clinical and Histopathological Study on Diagnostic Value of Total Renin

总肾素诊断价值的临床及组织病理学研究

• 批准号: 04454312
• 负责人: TSUCHIDA Yoshiaki
• 金额: $ 1.98万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454312/
• 关键词: Total Renin; Active Renin; Inactive Renin; Wilms' Tumor; Mesoblastic Nephroma; Multicystic Kidney; Radioimmunoassay; Immunohistochemistry; レニン; 腎腫瘍; 抗レニンモノクローナル抗体; ウィルムス腫瘍; 先天性間葉芽腎腫; 高血圧症

No tumor maker specific for Wilms'tumor has been known. Total renin consists of active and inactive forms, and it is known that inactive form of renin constitutes 70% to 90% of total renin in plasma. Since it was reported in 1985 that inactive renin can be a good marker for Wilms' tumor, we started to investigte whether or not total renin in plasma as determined by an RIA with monoclonal antibodies can become a much better marker for this tumor. As a preliminary study, we could confirm that an RIA system developed by Nishiura and his associates truly determines total renin concentrations, by a good correlation with data obtained withthe conventional enzymatic assay, and also by an immunohistochemical study ofmonoclonal antibodies which stained exclusively juxtaglomurular apparatuses of the kidney. We also found the upper border of the normal ranges of plasma total renin is 301 pg/ml in children. Total renin in plasma of 13 patients with Wilms' tumor ranged from 231 pg/ml to 1,289 pg/ml, and 9 out of the 13 patients showed values beyond the upper border of the normal ranges. Postoperatively, plasma total renin decreased to the normal ranges in all 5 patients studied. However, there was no correlation between the plasma values and stages. Two patients each with congenital mesoblastic nephroma and multicystic dysplastic kidney showed exessively high values of total renin, which was also confirmed immunohistochemically. Total renin was normal in all 16 patients with neuroblastoma. Three of 5 cases of hepatoblastoma and one of 10 cases of teratoma showed high values of plasma total renin, and they were considered as examples of extrarenal renin production.

尚无已知的针对肾母细胞瘤的特异性肿瘤标记物。总肾素由活性形式和非活性形式组成，已知非活性形式的肾素占血浆中总肾素的70%至90%。自从 1985 年报道无活性肾素可以成为肾母细胞瘤的良好标记物以来，我们开始研究用单克隆抗体进行 RIA 测定的血浆中总肾素是否可以成为该肿瘤更好的标记物。作为一项初步研究，我们可以确认西浦及其同事开发的 RIA 系统确实可以测定总肾素浓度，该系统与传统酶测定获得的数据具有良好的相关性，并且还通过对仅对肾脏近肾小球装置进行染色的单克隆抗体进行免疫组织化学研究。我们还发现儿童血浆总肾素正常范围的上限为301 pg/ml。 13 名肾母细胞瘤患者血浆中总肾素的范围为 231 pg/ml 至 1,289 pg/ml，13 名患者中有 9 名的值超出正常范围的上限。术后，所有 5 名研究患者的血浆总肾素均降至正常范围。然而，血浆值和阶段之间没有相关性。两名先天性中胚层肾瘤和多囊性发育不良肾患者的总肾素值过高，这一点也得到了免疫组织化学的证实。所有 16 名神经母细胞瘤患者的总肾素均正常。 5例肝母细胞瘤中的3例和10例畸胎瘤中的1例显示出血浆总肾素的高值，并且它们被认为是肾外肾素产生的例子。

项目成果

Yoshiaki Tsuchida: "Renin production in congenital mesoblastic nephroma in comparison with that in Wilms'tumor" Pediatric Pathology. 13. 137-146 (1993)

Yoshiaki Tsuchida：“先天性中胚层肾瘤中肾素的产生与维尔姆斯肿瘤的比较”《儿科病理学》。

Komura M,Tsuchida Y,et al.: "Evaluation of the diagnostic value of total renin concentration as a tumor marker" J Jpn Soc Pediatr Surg. 28. 1157-1162 (1992)

Komura M，Tsuchida Y，等：“总肾素浓度作为肿瘤标志物的诊断价值的评估”J Jpn Soc Pediatr Surg。

Tsuchida Y,Honna T,et al.: "Renin production in congenital mesoblastic nephroma in comparison with that in Wilms' tumor. Honna T,et al" Pediatr Pathol. 13. 155-164 (1993)

Tsuchida Y、Honna T 等人：“先天性中胚层肾瘤中肾素的产生与肾母细胞瘤中的肾素产生比较。Honna T 等人”儿科病理。

古村 真、土田嘉昭、本名敏郎、他: "血漿総レニン濃度の腫瘍マーカーとしての診断的意義に関する基礎的評価" 日本小児外科学会雑誌. 28. 1157-1162 (1992)

Makoto Furumura、Yoshiaki Tsuchida、Toshiro Honna 等：“血浆总肾素浓度作为肿瘤标志物的诊断意义的基本评估”日本小儿外科学会杂志 28. 1157-1162 (1992)。

Tsuchida Y.,Honna T.,Nishiura M.et al: "Renin Production in congerital mesoblastic nephroma in comparisom with that in wilms'tumor" Pediathia pathology. 13. 155-164 (1993)

Tsuchida Y.、Honna T.、Nishiura M.等人：“先天性中胚层肾瘤中肾素的产生与肾母细胞瘤的比较”Pediathia 病理学。