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Development of New Solution for Preservation of the Liver Graft.

开发保存肝移植物的新解决方案。

基本信息

批准号：
05557076
负责人：
TSUCHIDA Yoshiaki
金额：
$ 4.67万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557076/
关键词：
Liver Transplantation Solution for Preserving the Liver Graft Electrolyte Components of the Extra-cellular Fluid Liver Transplantation in Rats Liver Transplantation in Monkeys 臓器保存液ラット hydroxyethyl starch

项目摘要

The University of Wisconsin (UW) Solution has been shown to preserve all intraabdominal organs sucessfully, especially the liver, for 24 to 48 hours, and liver transplantation after extended preservation with this solution is performed at many institutions and hospitals at present. However, UW solution has a complex composition, must be stored in a refrigerator, and is expensive.We employed the use of inexpensive TOM-2H solution for simple cold storage of the liver (Iwanaka T,Kawarasaki H,Tsuchida Y,et al : Experimental Orthotopic Liver Transplantation Using TOM-2H Solution, in Which the Major Anion is Gluconate. Transplant. Proc.24 : 1602-1604,1992) , but we found the viscosity of TOM-2H solution is very high and the electrolyte components are of the intracellular type. During the year period of 1993 and 1994, we synthesized an improved TOM solution, a modification of TOM-2H solution, which has low viscosity and extracellular type electrolytes components. The results of experimental orthotopic liver transplantation in rats after 24 hours of simple cold storage using this modified TOM solution were exellent compared with the experiments using TOM-2H soluton and as good as those results using UW solution, in terms of wet liver weight after preservation, ATP and pH values after 1 hour preservation, survivals of the recipient rats, histologic changes of the liver, body weight of the recipients, liver function tests of the recipients, etc.In 1994 and 1995, we also performed the same experiments using 8 sets of monkeys. However, because of some surgical technical problems, we were unable to obtain sufficient number of survivors to draw conclusions.

威斯康星大学（UW）的溶液已被证明可以成功地保存所有腹内器官，特别是肝脏24至48小时，目前许多机构和医院都在使用该溶液进行延长保存后的肝移植。然而，UW溶液成分复杂，必须存放在冰箱中，而且价格昂贵。我们使用廉价的TOM-2H溶液进行肝脏的简单冷藏(Iwanaka T,Kawarasaki H，Tsuchida Y等：使用TOM-2H溶液进行实验性原位肝移植，其中主要阴离子为葡萄糖酸盐。移植。Proc.24: 1602-1604,1992)，但我们发现TOM-2H溶液的粘度非常高，电解质成分为胞内型。在1993年和1994年期间，我们合成了一种改进的TOM溶液，这是一种对TOM- 2h溶液的改性，它具有低粘度和胞外型电解质成分。在保存后的肝湿重、保存1小时后的ATP和pH值、受体大鼠的存活率、肝脏组织学变化、受体体重、受体肝功能指标等方面，经改良的TOM溶液简单冷藏24小时后的大鼠原位肝移植实验结果均优于TOM- 2h溶液，也优于UW溶液。在1994年和1995年，我们也用8组猴子进行了同样的实验。然而，由于一些手术技术问题，我们无法获得足够数量的幸存者来得出结论。