Analysis of transformation mechanism from differentiated to undifferentiated thyroid cancer

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1. Analysis of genetic levelMutation of p53 gene was studied for undifferentiated (anaplastic) and differentiated (papillary) thyroid carcinoma. An abnormal basic sequence was found only in anaplastic cancer specimens. This result suggests that mutation of p53 may trigger the anaplastic transformation from the papillary thyroid cancer.2. Analysis of protein levelHuman monoclonal antibody prepared from cervical lymph nodes taken from patients with anaplastic thyroid cancer with differentiated thyroid cancer specimens. Antigen recognized by this monoclonal antibody was even expressed in differentiated thyroid cancer. This monoclonal antibody may be useful tool for studying the mechanism of anaplastic transformation of thyroid cancer.3. Analysis of cell kineticsFour anaplastic thyroid cancer cell lines and 3 poorly differentiated cancer cell lines were established. K-199, an anaplastic cancer cell line, secretes granulocyte-colony stimulating factor (G-CSF), which may play some role in the growth of anaplastic thyroid cancer with an autocrine or paracrine mechanism. By adding a differentiated thyroid cancer cell line provided by fellow investigators, we were able to prepare a cell panel, which covered both highly and poorly differentiated and also undifferentiated thyroid cancer, ncluding differentiated thyroid cancer cell line, presented by other investigator, we can prepare the cell panel of thyroid cancer covering from differentiated to poorly and further undifferentiated thyroid cancer. As shown in genetic study, abnormal expression of p53 relationg anaplastic transformation was also found in this cell panel.