Immunological prevention of type 1 diabetes using GAD65-encording DNA vaccine

使用编码 GAD65 的 DNA 疫苗对 1 型糖尿病进行免疫预防

• 批准号: 20591056
• 负责人: KOBAYASHI Tetsuro
• 金额: $ 3万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591056/
• 关键词: GAD65; 1型糖尿病; 膵島炎; GAD抗体; アミラーゼ抗体; GAD; β-細胞; エピトープ

Preventive effect of human glutamic acid decarboxylase (GAD) 65 (GAD65) vaccination for type 1 diabetes was examined using NOD mice model. Subcutaneous electropolation of site-specific GAD65 fragments including GAD65 (1-83aa : N-GAD65), GAD65 (244-443aa : Center-GAD65) and GAD65 (443-585aa : C-GAD65) were done to 4 week female NOD mice.Specific T cell responses to N-GAD65, Center-GAD65 and C-GAD65 were observed to respective GAD65 fragments in electropolated NOD mice. However, occurrence rate of diabetes in GAD65 vector- or empty vector- electropolated groups did not reach significant levels during 30 weeks.Human GAD65 different from mice GAD65 may explain a failure to prevent the occurrence of diabetes by human site-specific GAD65 vaccination.

采用NOD小鼠模型研究了人谷氨酸脱羧酶（GAD）65（GAD 65）疫苗对1型糖尿病的预防作用。包括GAD 65的位点特异性GAD 65片段的皮下电极化（1- 83 aa：N-GAD 65）、GAD 65用N-GAD 65（244- 443 aa：Center-GAD 65）和C-GAD 65（443- 585 aa：C-GAD 65）对4周龄雌性NOD小鼠进行电极化，观察N-GAD 65、Center-GAD 65和C-GAD 65对相应GAD 65片段的特异性T细胞应答。然而，在30周期间，GAD 65载体或空载体电介导的组中糖尿病的发生率未达到显著水平，人GAD 65不同于小鼠GAD 65可能解释了人位点特异性GAD 65疫苗接种未能预防糖尿病的发生。

项目成果

Immunization with thyroglobulin induces Graves'-like disease in mice.

• DOI: 10.1677/joe-09-0089
• 发表时间: 2009-08
• 期刊: The Journal of endocrinology
• 作者: Endo T;Kobayashi T
• 通讯作者: Kobayashi T

HSP 10 is a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes.

• DOI: 10.1016/j.bbrc.2009.06.009
• 发表时间: 2009-08
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Soichi Takizawa;T. Endo;Wanjia Xing;Shoichiro Tanaka;Masashi Takahashi;Tetsuro Kobayashi

RIG-I-andMDA5-Initiated Innate Immunity Linked With Adaptove Immunity Acceleratesβ-Cell Death in Fulminant Type 1 Diabetes

RIG-I-和MDA5-启动的先天免疫与适应性免疫相关，加速暴发性1型糖尿病中的β细胞死亡

• 发表时间: 2011
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: K. Aida;Y. Nishida;S. Tanaka;T. Maruyama;A. Shimada;T. Awata;M. Suzuki;H. Shimura;S. Takizawa;M. Ichijo. D. Akiyama;F. Furuya;A. Kawaguchi;M. Kaneshige;J. Itakura;H. Fujii;T. Endo;and T. Kobayashi
• 通讯作者: and T. Kobayashi

Pathlogical changes in the pancreas of fulminant type 1 diabetes and slowly progressive insulin-dependent(type 1)diabetes

暴发性1型糖尿病和缓慢进展型胰岛素依赖型（1型）糖尿病的胰腺病理改变

• 发表时间: 2010
• 作者: Ando H;Ushijima K;Kumazaki M;Takamura T;Yokota N;Saito T;Irie S;Kaneko S;Fujimura A;T.Kobayashi
• 通讯作者: T.Kobayashi

Enterovirus infection, CXC chemokine ligand 10 (CXCL10), and CXCR3 circuit: a mechanism of accelerated beta-cell failure in fulminant type 1 diabetes.

• DOI: 10.2337/db09-0091
• 发表时间: 2009-10
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: Tanaka S;Nishida Y;Aida K;Maruyama T;Shimada A;Suzuki M;Shimura H;Takizawa S;Takahashi M;Akiyama D;Arai-Yamashita S;Furuya F;Kawaguchi A;Kaneshige M;Katoh R;Endo T;Kobayashi T
• 通讯作者: Kobayashi T