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Immunohistchemical and molecular biological studies on severe mucosal pathology of the upper airway.

上呼吸道严重粘膜病理学的免疫组织化学和分子生物学研究。

基本信息

批准号：
04454431
负责人：
OHYAMA Masaru
金额：
$ 3.84万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

1.An in vitro culture system in which human respiratory epithelial cells can grow and differentiate into both ciliated and secretory cells was established. Using these floating cultured cells, the studies on the regulation of cell differentiation due to some cytokines as well as the ciliary metachronism due to exogenous ATP,endotoxin and macrolide antibiotics were performed.2.It was revealed that IL-1beta is predominant cytokine in sinus fluid of chronic sinusitis and is produced by polymorphonuclear neurophils, induced the expression of ICAM-1 and ELAM-1 on endothelial cells from the results of both immunohistchemical and biochemical studies, in addition to IL-1beta mRNA detection by in situ hybridization.3.Lectin histochemisty showed that both increased staining of glycoproteins such as fucose and sialic acid and diferent composition of these terminal carbohydrates in addition to dominantly reduced intensity of saccharide chain of mannose were observed in sinusitis mucosa. There were increased sttaining of PNA in well differntiated type and it of SNA in anaplastic type of laryngeal squamous cell carcinoma.4.A predominance of HPV-11 was detected by PCR among recurrent airway papillomatosis (RAP). The presence of antibodies against the recombinant E6 and L2 proteins of HPV-11 subcloned were demonstrated serologically in RAP patients. The E6 and E7 genes of HPV-16 were transcribed from the P97 promotor and were regulated by cellular transcriptional factors and viral E2 gene product. It was showed that HPV-16 E2 protein had the ability to trans-activate the heterogenous promotor at the same level of BPV-1 E2 protein in the in vivo and in vitro experiments.5.Baloon laserthermia equippment was deviced successfully and was applied into the patients with nasal allergy and maxillary cancers showing the fairly good clinical results.

1.建立了人呼吸道上皮细胞的体外培养体系，在此体系中，人呼吸道上皮细胞既能生长，又能分化为纤毛细胞和分泌细胞。利用这些细胞进行了细胞因子对细胞分化的调节以及外源性ATP、内毒素和大环内酯类抗生素对纤毛异时性的影响的研究。2. IL-1 β是慢性鼻窦炎窦液中的主要细胞因子，由多形核嗜神经细胞产生。从免疫组织化学和生物化学研究的结果来看，除了IL-3.凝集素组化结果显示，两种细胞均出现岩藻糖、唾液酸等糖蛋白染色增强，在鼻窦炎粘膜中，除了甘露糖的糖链强度显著降低外，还观察到这些末端碳水化合物的减少。高分化型喉鳞状细胞癌中PNA的阳性率明显增高，间变性型喉鳞状细胞癌中SNA的阳性率明显增高。4. PCR检测发现复发性气道乳头状瘤病（RAP）中HPV-11的阳性率明显增高。RAP患者血清学上显示存在针对HPV-11亚克隆的重组E6和L2蛋白的抗体。HPV-16的E6和E7基因由P97启动子转录，受细胞转录因子和病毒E2基因产物的调控。体内、外实验结果表明，HPV-16 E2蛋白具有与BPV-1 E2蛋白相同水平的反式激活异源启动子的能力。5.成功研制了Baloon激光热疗仪，并应用于鼻腔变态反应和上颌窦癌的治疗，取得了较好的临床效果。