Clinical epidemiology of female laryngeal cancer in China. -with a special attention to clinicopathological, cytochemical and molecular biological studies.-

中国女性喉癌临床流行病学。

• 批准号: 07042009
• 负责人: OHYAMA Masaru
• 金额: $ 2.88万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07042009/
• 关键词: female patient; laryngeal cancer; Blinkman index; malignant HPV; benign HPV; P53; neck metastasis; DF3 antigen; Lectin SNA; 声門上部癌; レクチン組織化学; EBV; HPV; p53

The present study concentrates on the clinicoepidemiological and virological aspects of female patient with laryngeal cancer (FLC) in Shenyang with emphasis on specific environmental factor as they exist in this district.1. Clinicoepidemiological study : Two hundred sixty five patients who were composed of 181 male patients with laryngeal cancer (MLC) and 84 FLC were analyzed. In FLC,tumor was more frequently involved in the supraglottic region and relatively higher habits of tobacco smoking showing average value of Blinkman index of 634.7, while those of 768.6 in MLC.2. HPV DNA and P53 gene detection studies : One hundred two LC-speciments taken from 73 MLC and 29 FLC were studied. HPV-16 was detected in 22 cases (36.7%), HPV-18 in 10 cases (16.7%), HPV-33 in one case (1.7%), while HPV-6 was detected in 5 cases (8.3%) and HPV-11 in 2 cases (3.3%). Both HPV-16 and 6 were found in 8 cases (13.3%) and HPV-18 and 6 were detected in 12 cases (20.0%). Overall the prevalence of malingnant HPV was 52.0% in 53 of 102 cases. P53 in the LC specimens was detected in 60 of 102 cases (58.8%). There is a significant relation between the prevalence of P53 in the specimens and an incidence of neck metastasis.3. The studies on expression of mucin antigen and glycoconjugates : Fifty one specimen of LC and 49 of dysplastic squamous epithelia (DSE) taken from the patients in our university hospital were examined. SNA (Smabucus nigra aggllutinin) showed more intensively stained cytoplama of carcinoma cells as compared to those of the DSE.DF3 antigen (mammary-type apomucin) and Sialyl-Tn antigens were more frequently seen in carcinoma than in the DSE.They may be an effective marker for squamous cell carcinoma of the larynx.

本研究对沈阳市女性喉癌患者的临床流行病学和病毒学特征进行了研究，重点分析了该地区存在的特殊环境因素。临床流行病学研究：对265例男性喉癌患者和84例喉癌患者进行分析。肿瘤多累及声门上区，吸烟习惯较高，BLinkman指数平均值为634.7，MLC.2为768.6。HPV DNA和P53基因检测研究：对73例MLC和29例FLC的102例LC标本进行检测。HPV-16阳性22例(36.7%)，HPV-18阳性10例(16.7%)，HPV-33阳性1例(1.7%)，HPV-6阳性5例(8.3%)，HPV-11阳性2例(3.3%)。HPV-16和HPV-6双阳性8例(13.3%)，HPV-18和HPV-6双阳性12例(20.0%)。总体而言，102例患者中53例的HPV感染率为52.0%。102例LC标本中有60例(58.8%)P53蛋白阳性。标本中P53蛋白的表达与颈转移的发生率有明显的相关性。粘蛋白抗原和糖偶联物表达的研究：对51例LC和49例异型增生鳞状上皮(DSE)标本进行检测。与DSE相比，SNA(Smabucus Ngra Aglutinin)的癌细胞胞浆染色更深，DF3抗原(乳腺型无粘蛋白)和sialyl-TN抗原在癌中的出现频率高于DSE，它们可能是喉鳞状细胞癌的有效标志物。

项目成果

T.Ioh,S.Yonezawa,M.Nomoto,K.Ueno,Y.S.Kim,and E.Satoh: "Expression of mucine antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx."

T.Ioh、S.Yonezawa、M.Nomoto、K.Ueno、Y.S.Kim 和 E.Satoh：“粘液抗原和 Lewis X 相关抗原在咽喉癌和发育不良中的表达。”

T. Itoh, S. Yonezawa, M. Nomoto K. Ueno, Y. S. Kim, and E. Sato: "Expression of mucin antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx." Pathology International. 46. 646-655 (1996)

T. Itoh、S. Yonezawa、M. Nomoto K. Ueno、Y. S. Kim 和 E. Sato：“粘蛋白抗原和 Lewis X 相关抗原在咽喉癌和发育不良中的表达。”

T.Itoh, S.Yonezawa, M.Nomoto, K.Ueno, Y.S.Kim, E.Satoh: "Expression of mucine antigens and Lewis X-related antigens in Carcinomas and dysplasia of the pharynx and larynx."

T.Itoh、S.Yonezawa、M.Nomoto、K.Ueno、Y.S.Kim、E.Satoh：“粘液抗原和 Lewis X 相关抗原在咽喉癌和发育不良中的表达。”

王振海: "喉頭癌組織における糖鎖発現様式" 日本耳鼻咽喉科学会会報. 98. 1781-1787 (1995)

王振海：“喉癌组织中的聚糖表达模式”日本耳鼻喉科学会会报 98. 1781-1787 (1995)。

Zhen Hai Wang: "Expression of Glycoconjugates in Laryngeal Cancer." Journal of Otolaryngology of Japan. 98. 1781-1787 (1995)

王振海：“糖复合物在喉癌中的表达”。