Epidemiological studies on Juvenile laryngeal papillomatosis in Thailand.

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The existence of juvenile laryngel papilloma (JLP) is presently low. Reports in the literatures become rare over the past decade whereas its presentation in Thailand is still very common. The present study concentrates on the clinicoepidemiological and virological aspects of JLP in Thailand with emphasis on specific environmental factor as they exist in this country.1. Clinicoepidimiological aspectsTwo hundred fifty nine patients with JLP who have been treated in the ENT clinics in Thailand and 62 cases who were collected by the members of this research group could be analyzed in the clinical study.The clinical onset varies and is from the newborn to puberty ; 89% of the cases are diagnosed before the age of 6 years. They are most frequently seen in the firstly delivered baby. The majority of the population lives in upcountry areas in Thailand where a certain level of poverty and bad hygienic cicumstances contribute to higher frequency of infectious diseases.The glottic region is frequ … More ently involved in these JLP cases and in the majority of these cases, hoarseness starts already when children begin to speak, followed by complete aphonia and dyspnea.2. Virological studyTwenty nine samples of JLP were studied molecular-biologically. Both dot and southern blot hybridization techniques, and PCR revealed the type 11 of HPV in 23 cases and the type 6 in 3 cases. However, type 16 and type 18 could not be detected in any of these cases. These results indicate that type 11 of HPV is the most frequent type of JLP which occurs in Thai children, although we have found frequently HPV 6 in north east Asia or western countries. A relationship between the clinical features of JLP and the HPV type is not clear.We could made successfully recombinant HPV-L-2 protein by HPV-11 DNA cloning. It is found that this recombinant L-2 protein could be responded markedly to the sera which were collected Thai children with JLP type 11.Using this anti L-2 antibody, clinical application for the serological diagnosis to the JLP patients and the related cases in the field works will be expected. Less