Immunohistochemical Detection of Oncogene Products in Tumor cells in the Oral Cavity and Salivary Glands.

用免疫组织化学方法对口腔和唾液腺肿瘤细胞中的癌基因产物、EGF受体和PCNA进行了评价。以同样的方法评价了鼠颊袋的实验性癌，并对热疗的组织效应进行了病理和免疫组织化学研究。在正常口腔黏膜和良性肿瘤中，基底细胞核可见PCNA染色，基底和棘层细胞膜可见EGF-r染色。在高分化鳞状细胞癌（SCC）中，PCNA阳性细胞核位于基底和棘层，EGF-r阳性细胞分散在基底和棘层。在低分化SCC中，多数肿瘤细胞为PCNA阳性核，EGF-r阴性核。在SCC中，PCNA阳性细胞无EGF-r染色。P53染色仅限于细胞核，约50%的口腔SCC呈阳性，P53阳性肿瘤细胞的频率与组织学恶性相关。在唾液腺肿瘤中，c-erbB-2存在于极少数恶性病例的细胞膜中。正常唾液腺腺泡和腺管几乎未见PCNA阳性细胞。多形性腺瘤中PCNA阳性细胞约占5%，高恶性涎腺肿瘤中PCNA阳性细胞约占20%以上。鼠颊袋癌，约15%肿瘤细胞PCNA阳性。热疗后肿瘤细胞呈减少和凋亡的特征。肿瘤中可见血管扩张及炎性细胞浸润。热疗后肿瘤细胞角蛋白染色减少，PCNA阳性细胞核数量明显减少，肿瘤风暴中tenascin染色增强。癌基因产物和EGF-r在实验癌中呈阴性。

Oncogene products, EGF receptor and PCNA were evaluated immunohistochemically in tumor cells from the oral cavity and salivary glands. Experimental carcinomas in hamster cheek pouch was also evaluated same manner and tissue effects for thermotherapy were studied pathologically and immunohistochemically.In normal oral mucosa and benign tumors, PCNA staining was found in basal cell nuclei, and EGF-r in cell membrane of basal and spinous cell layrs. In well differentiated squamous cell carcinoma (SCC), PCNA positive nuclei were located basal and spinous cells and EGF-r positive cells were scattered in basal and spinous layr. In poorly differentiated SCC, PCNA positive nuclei were found most of tumor cells, while EGF-r was negative. In SCC, PCNA positive cell was devoid of EGF-r staining. P53 staining is limited to nuclei and about 50% of oral SCC were positive and frequency of p53 positive tumor cells was correlated to histological malignancy. In salivary gland tumors, c-erbB-2 was found in cell membrane of very few malignant cases. Normal salivary gland showed almost no PCNA postive cells in acini and ducts. About 5% of tumor cells in pleomorphic adenoma showed positive PCNA staining and over 20% in higher malignant salivary gland tumors. Experimental carcinoma in hamster cheek pouch, about 15% of tumor cells were positive for PCNA.After thermotherapy, tumor cells were characterized by diminsh and existance of apoptosis. Dilatation of blood vessels and infiltration of inflammatory cell were seen in tumor strom. Keratin staining in tumor cells were decreased, PCNA positive nuclei were remarkably decreased in number, and tenascin staining in tumor storom was enhanced after the thermotherapy. Oncogene products and EGF-r showed negative in experimental carcinoma.