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Immunohistochemical Detection of Oncogene Products in Tumor cells in the Oral Cavity and Salivary Glands.

口腔和唾液腺肿瘤细胞中癌基因产物的免疫组织化学检测。

基本信息

批准号：
04454458
负责人：
MORI Masahiko
金额：
$ 3.39万
依托单位：
Asahi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454458/
关键词：
Oncogene protoncogene oral mucosa salivary gland tumor EGF receptor PCNA EGF reccptor

项目摘要

Oncogene products, EGF receptor and PCNA were evaluated immunohistochemically in tumor cells from the oral cavity and salivary glands. Experimental carcinomas in hamster cheek pouch was also evaluated same manner and tissue effects for thermotherapy were studied pathologically and immunohistochemically.In normal oral mucosa and benign tumors, PCNA staining was found in basal cell nuclei, and EGF-r in cell membrane of basal and spinous cell layrs. In well differentiated squamous cell carcinoma (SCC), PCNA positive nuclei were located basal and spinous cells and EGF-r positive cells were scattered in basal and spinous layr. In poorly differentiated SCC, PCNA positive nuclei were found most of tumor cells, while EGF-r was negative. In SCC, PCNA positive cell was devoid of EGF-r staining. P53 staining is limited to nuclei and about 50% of oral SCC were positive and frequency of p53 positive tumor cells was correlated to histological malignancy. In salivary gland tumors, c-erbB-2 was found in cell membrane of very few malignant cases. Normal salivary gland showed almost no PCNA postive cells in acini and ducts. About 5% of tumor cells in pleomorphic adenoma showed positive PCNA staining and over 20% in higher malignant salivary gland tumors. Experimental carcinoma in hamster cheek pouch, about 15% of tumor cells were positive for PCNA.After thermotherapy, tumor cells were characterized by diminsh and existance of apoptosis. Dilatation of blood vessels and infiltration of inflammatory cell were seen in tumor strom. Keratin staining in tumor cells were decreased, PCNA positive nuclei were remarkably decreased in number, and tenascin staining in tumor storom was enhanced after the thermotherapy. Oncogene products and EGF-r showed negative in experimental carcinoma.

应用免疫组化方法检测口腔癌和涎腺癌细胞癌基因产物、EGF受体和PCNA的表达。在正常口腔粘膜和良性肿瘤中，PCNA染色见于基底细胞核，EGF-r染色见于基底细胞层和棘细胞层细胞膜。在高分化鳞癌中，PCNA阳性细胞核位于基底层和棘层，EGF-r阳性细胞散在分布于基底层和棘层。在低分化鳞癌中，PCNA阳性细胞核多见，EGF-r阴性。SCC中PCNA阳性细胞无EGF-r染色。P53仅表达于细胞核，约50%的口腔鳞癌为阳性，且P53阳性细胞的频率与组织学恶性程度相关。在涎腺肿瘤中，c-erbB-2仅见于极少数恶性肿瘤的细胞膜。正常涎腺腺泡和导管内几乎无PCNA阳性细胞。PCNA在多形性腺瘤中的阳性表达率约为5%，在恶性程度较高的涎腺肿瘤中的阳性表达率超过20%。实验性金黄地鼠颊囊癌中，约15%的癌细胞PCNA阳性，热疗后癌细胞数量减少，有凋亡细胞存在。瘤栓内血管扩张，炎细胞浸润。热疗后肿瘤细胞角蛋白染色减少，PCNA阳性细胞核数量明显减少，瘤腔中生腱蛋白染色增强。癌基因产物和EGF-r在实验性肿瘤中均为阴性。