Promotion effect of lymchocytic thyroiditis in thyroid carcinogenesis by activation of RET protoncogene

淋巴细胞性甲状腺炎RET原癌基因激活对甲状腺癌发生的促进作用

• 批准号: 11670189
• 负责人: OKAYASU Isao
• 金额: $ 2.5万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670189/
• 关键词: thyroid cancer; RET protoncogene; thyroiditis; Ras gene; carcinogenesis

1. RET protein overexpression was immunohistochemically revealed in the sequence order of normal follicular epithelium, follicular carcinoma, follicular adenoma, poorly differentiated papillary carcinoma, lymphocytic thyroiditis and well differentiated papillary carcinoma.Increased expression of RET protein was stronger in well differentiated papillary carcinoma with lymphocytic thyroiditis in the background than without thyroiditis, although the difference did not reach the significance.2. RT-PCR-Southern blot hybridization showed activation of RED protoncogene in every lesion, possibly due to contamination of endothelium within the thyroid tissue.3. Point mutations of ras gene was rare in papillary carcinomas, although frequent N-ras mutations was found in follicular carcinomas.In conclusion, RET protein expression has an intimate relationship to the development of lymphocytic thyroiditis and thyroid papillary carcinoma. However, it is suggested that another markers are necessary to confirm the promotion effect of lymphocytic thyroiditis in thyroid carcinogenesis.

1.结果：1.免疫组化显示RET蛋白的过表达顺序为：正常滤泡上皮、滤泡癌、滤泡腺瘤、低分化乳头状癌、淋巴细胞性甲状腺炎、高分化乳头状癌，高分化乳头状癌伴淋巴细胞性甲状腺炎组RET蛋白的表达高于无淋巴细胞性甲状腺炎组，但差异无统计学意义. RT-PCR-Southern杂交显示，甲状腺组织中RED原癌基因均被激活，这可能与甲状腺组织内内皮细胞污染有关. ras基因点突变在乳头状癌中少见，而N-ras基因点突变在滤泡状癌中常见，RET蛋白表达与淋巴细胞性甲状腺炎和甲状腺乳头状癌的发生发展密切相关。然而，这表明，另一个标志物是必要的，以确认在甲状腺癌的发生淋巴细胞性甲状腺炎的促进作用。

项目成果

Yanagisawa N, Mikami T, Koike M, Okayasu I.: "Enhanced cell kinetics, p53 accumulation and high p21WAF1 expression in chronic cholecystitis : comparison with background mucosa and gallbladder carcinomas."Histopathology. 36. 54-61 (2000)

Yanagisawa N、Mikami T、Koike M、Okayasu I.：“慢性胆囊炎中增强的细胞动力学、p53 积累和高 p21WAF1 表达：与背景粘膜和胆囊癌的比较。”组织病理学。

Komatsu H, Okayasu I, et al: "Immunohistochemical detection of human gastro intestinal glutathione peroxidase in normal tissues and cultured cells with novel mouse monedonal antibodies"J.Histochem.Cytochem.. (in press). (2001)

Komatsu H、Okayasu I 等人：“用新型小鼠单克隆抗体对正常组织和培养细胞中的人胃肠道谷胱甘肽过氧化物酶进行免疫组织化学检测”J.Histochem.Cytochem..（出版中）。

Yanagisawa N,Okayasu I. et al: "Enhanced Cell kinetics, p53 accumulation and high p21 WAPI expression inchronic cholecystitis : comparison with background mucosa adn gallbladder carcinomas"Histopathology. 36. 54-61 (2000)

Yanagisawa N,Okayasu I. 等人：“增强的细胞动力学、p53 积累和高 p21 WAPI 表达慢性胆囊炎：与背景粘膜和胆囊癌的比较”组织病理学。

Tanigawa H, Uesugi M, Mitomi H, Saigenji K, Okayasu I.: "Possible association of active gastritis, featuring accelerated cell turnover and p53 overexpression with cancer development at anastomosis after gastroje jenostomy."Am J Clin Pathol. 114(3). 354-36

Tanikawa H、Uesugi M、Mitomi H、Saigenji K、Okayasu I.：“活动性胃炎可能与胃肠造口术后吻合处癌症的发展有关，其特点是细胞更新加速和 p53 过度表达。”Am J Clin Pathol。

Tanigawa H,Okayasu I,et al: "Possible association of active gastritis featuring accelerated cell turnover and p53 overexpression with cancer development at anastomosis after gastrojejunostomy"Am.J.Clin.Pathol. 114(3). 354-363 (2000)

Tanikawa H、Okayasu I 等人：“以加速细胞更新和 p53 过度表达为特征的活动性胃炎与胃空肠吻合术后吻合处癌症发展的可能关联”Am.J.Clin.Pathol。