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核磁気共鳴装置による運動中の筋代謝の非観血的及び連続測定

使用核磁共振设备无创连续测量运动过程中的肌肉代谢

基本信息

批准号：
04454588
负责人：
YOSHIDA Takayoshi
金额：
$ 3.2万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

It has been well documented that ^<31>phosphorus-nuclear magnetic resonance spectroscopy (^<31>P-MRS) has been used as a non invasive measurement of changes in concentrations of adenosine 5ユ^<**> triphosphate (ATP), phosphocreatine (PCr) and inorganic phosphate (Pi), as well as the intramuscular pH both during and after exercise. Splitting of the Pi peak into two peaks has been observed during exercise and is attributed to two different pH distributions in exercising muscle (high pH and low pH). Previously, we reported that the two split Pi peaks showed different time courses at the onset of exercise and during recovery, the high-pH Pi increased promptly at the onset of exercise and disappeared rapidly after exercise, while the low pH Pi peak increased gardually with after a delay of about 60 sec at the onset of exercise and decreased over a longer period after exercise. To assess the physiological mechanism for Pi spiltting, we have studied the effect of exercise intensity, inspired oxygen concentration and/or cooling down on Pi peak splitting.Pi peak splitting is observed dependent on the exercise intensity. In other words, the splitting was observed when the exercise energy metabolism can not solely derive from aerobic metabolism, above 1.0 value of Pi/PCr ratio, suggesting anaerobic glycolysis recruitment. Furthemore, low-pH Pi peak much more documented when hypoxic gas inhalation, suggesting anaerobic glycolysis documented. When active recovery was performed after the exercise, the low-pH value returned more quickly to the pre-exercise level than during passive recovery. The quick disappearance of the low-pH Pi peak can be explained by differences in removal of lactate by blood flow rate maintained during active recovery, and the subsequent acceleration of glycolysis in the FT fibers.

已有文献证明，磷-核磁共振波谱(P-MRS)已被用于无创性地测量运动过程中和运动后三磷酸腺苷(ユ)、磷酸肌酸(Pcr)和无机磷(PI)浓度的变化，以及肌肉内的pH变化。在运动过程中观察到等电点峰分裂成两个峰，这归因于运动肌肉中两种不同的pH分布(高pH和低pH)。在此之前，我们报道了两个分裂的PI峰在运动开始和恢复期间呈现不同的时间进程，高pH PI在运动开始时迅速增加，运动后迅速消失，而低pH PI峰值在运动开始时延迟约60s后逐渐增加，并随着运动后较长时间的推移而下降。为了探讨PI溢出的生理机制，我们研究了运动强度、吸氧浓度和/或降温对PI峰分裂的影响。也就是说，当运动能量代谢不能完全来源于有氧代谢时，出现分裂，高于PI/PCR值1.0，提示无氧糖酵解补充。此外，当吸入低氧气体时，低pH等电点峰值更多地被记录下来，提示有记录的厌氧糖酵解。当运动后进行主动恢复时，低pH值恢复到运动前水平的速度比被动恢复时更快。低pH等电点峰的迅速消失可以解释为在主动恢复过程中维持的血流速度对乳酸的清除差异，以及随后FT纤维中糖酵解的加速。