Identification of new genes from YAC clones involved in cell development in the HLA region

我们利用YAC、粘粒和噬菌体载体克隆了人的MHC（HLA）基因，通过排列它们的重叠群构建了物理图谱，并通过cDNA分离和基因组测序分析寻找了新的表达基因。在与小鼠t复合体区域对应的II类区域的着丝粒侧，鉴定了8个新基因，即HSET（一种驱动蛋白样基因）-HKE 2（鸟嘌呤核苷酸解离刺激因子样基因）-HKE 3（核糖体S18亚基基因）-HKE 6（一种酒精脱氢酶样基因）-HKE 4（激肽原样基因）-RXRB（类维生素A X受体B基因）-HKE 5（未知基因）-COL 11 A2（α 2胶原X1）。一个未鉴定的400 kb区域，包含II类和III类区域之间的连接，4个新基因，TN-X（一种生腱蛋白样基因），（非酶糖基化蛋白终产物的受体基因），HOX12（PBX家族同源框基因）和Notch 3（属于Notch家族的小鼠int-3基因的人类同源物）以从三聚体侧到着丝粒侧的顺序鉴定。RXRB，HOX 12和Notch 3基因是负责一些胚胎和细胞发育的候选基因。这些基因的进一步鉴定对于阐明胚胎发育阶段细胞调控的分子机制具有重要意义。

We have cloned the human MHC (HLA) region by YAC,cosmid and phage vectors, constructed the physical map by lining up their contigs and searched for new expressed genes by cDNA isolation and genomic sequencing analysis. On the centromeric side of the class ll region which corresponds to the mouse t complex region, 8 new genes were identified, namely HSET (a kinesin-like gene) -HKE2 (guanine nucleotide dissociation stimulator-like gene) -HKE3 (a ribosome S18 subunit gene) -HKE6 (a alcohol dehydrogenase-like gene) -HKE4 (a kininogen-like gene) -RXRB (a retinoid X receptor b gene) -HKE5 (an unknown gene) -COL11A2 (a a2 collagen X1) from the centromeric side. An uncharacterized 400 kb region harboring the junction between the class ll and class lll regions, 4 new genes, TN-X (a tenascin-like gene), RAGE (a receptor gene for nonenzymatically glycosylated protein end product), HOX12 (a PBX-family homeobox gene) and Notch3 (a human homologue of the mouse int-3 gene belonging to a Notch family) were identified in the order of from the teromeric to centromeric sides. The RXRB,HOX12 and Notch3 genes are candidate genes responsible for some embryonic and cell development. Further characterization of these genes will be very important to elucidate the molecular mechanism of cell regulation at the embryonic development stage.