Genome-wide identification of genes for anorexia and their cascade analysis on disease development by functional study

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An eating disorder is a mental disorder which sharply increased since the late 1990s for people of young age. Its treatment strategies or prognostic methods, however, have yet to be established. In the study described here, we first identified susceptible genes of the disease, based on our genome-wide association analysis with microsatellite markers. To the identified susceptible genes, we applied our high-sensitivity yeast two-hybrid method, followed by immunoprecipitation analysis in mammalian cells. Through these analyses, we found dozens of disease-related interactors, identities of which suggest molecular function of the susceptible genes. We further analyzed one of the susceptible genes by protein-protein interaction assays in details and identified an 83 amino-acid region which is important for both molecular interactions and cellular localization and is, therefore, a good candidate as a drug development target.