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Study on Thrombolysis Using Small Ultrasound Vibration.

小超声振动溶栓研究。

基本信息

批准号：
04807064
负责人：
BEPPU Shintaro
金额：
$ 1.28万
依托单位：
Osaka University (1994)National Cardiovascular Center Research Institute (1992-1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807064/
关键词：
coronary artery thrombus recanalization ultrasound ultrasonic disruption catheter

项目摘要

It has been revealed that blood clot made in vitro was broken by ultrasound radiation from the ultrasound tip as thin as 3mm in diameter, in which the ultrasound frequency was 20KHz and the power was 1 Watt. Several self-made thin transducer was examined its power in a water bath. Direction and magnitude of the ultrasound power in the water could be visualized using solution diffused by microbubbles. After adjusting the ultrasound frequency to be maximize its effect, the transducer was applied to examine whether the transducer could enhance the thrombolysis in vitro. Dry weight of the clot was less by combination use of ultrasound radiation and Urokinase than that by sole use of Urokinase. This ultrasound effect is enhanced under presence of microbubbles, probably due to cavitation phenomenon. Long-life microbubbles are needed, while ordinal microbubbles are easy to be solved into water or blood. We developed a new agent of stable microbubbles, which was made from dodecafluoropentane. Its durability was examined in canines by echocardiography. The solution of dodecafluoropentane was injected into the peripheral vein and the myocardial opacification was evaluated. Using a dosage over 0.5ml/kg, the myocardium was clearly opacified for long period as over 10 to 50 minutes. Weconclude that the combination use of microtransducer of ultrasound and microbubbles will enhance the thrombolysis in vivo.

据透露，体外制作的血凝块被来自直径为3毫米的细超声波尖端的超声波辐射破坏，其中超声波频率为20KHz，功率为1瓦。几种自制的薄型换能器在水浴中测试了其功率。水中超声波功率的方向和大小可以通过微泡扩散的溶液来可视化。调整超声频率使其效果最大化后，应用换能器检测换能器是否能增强体外溶栓作用。联合使用超声辐射和尿激酶的凝块干重比单独使用尿激酶的凝块干重更轻。这种超声波效应在微泡存在的情况下会增强，这可能是由于空化现象。需要长寿命的微泡，而普通微泡很容易溶解到水或血液中。我们开发了一种新型稳定微泡剂，它是由十二氟戊烷制成的。通过超声心动图在犬科动物中检查其耐久性。将十二氟戊烷溶液注入外周静脉并评估心肌混浊。使用超过0.5ml/kg的剂量，心肌在超过10至50分钟的较长时间内明显浑浊。我们认为超声微换能器与微泡的联合使用将增强体内溶栓作用。