Drag Delivery System Using Microbubbles and Ultrasound

背景：微泡超声给药系统是近年来提出的一种新型给药系统。我们评价了超声辐照能否将微泡结合的荧光保留在靶组织内。方法：72只麻醉大鼠。制备了全氟碳暴露的声化葡萄糖白蛋白(PESDA)微泡，并与Cy^&lt；3Tm&gt；连续注射Pesda，并在几种超声和微泡设置中将超声辐射到心脏。结果：微泡的浓度不影响荧光强度，但微泡的注入速度、辐照时间和声功率对荧光强度有影响。结论：微泡和超声联合给药是可行的，其效果取决于微泡和超声的设置。

Background : Drug delivery system using microbubble and ultrasound is recently proposed. We evaluated whether fluorescence conjunct with microbubbles can be retained within the target tissue by ultrasound insonation.Methods : 72 anesthetized rats were used in this study. Perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles were made and conjugated with Cy^<3TM>, which is fluorescence. Cy^<3TM> PESDA was continuously injected and ultrasound was insonated to the heart in several ultrasound and microbubble settings. The heart was extracted and the intensity of fluorescence within the tissue was analyzed by offline software.Results : Concentration of microbubble did not influence the intensity of fluorescence; however, injection speed of microbubble, duration of insonation, and acoustic power influenced.Conclusion : Our results suggest that drug delivery using microbubble and ultrasound is possible and the effects depend on the settings of microbubble and ultrasound.