DEVELOPMENT OF THE NEW BIOCHEMICAL MARKER FOR EARLY DIAGNOSIS OF HEPATIC INFLOW IN SUFFICIENCY

开发用于早期诊断肝血流充足性的新生化标记物

基本信息

批准号：
04807083
负责人：
KOGURE Kimitaka
金额：
$ 1.22万
依托单位：
GUNMA UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

Hepatic vascular inflow occlusion and complete hepatic vascular exclusion have often been employed in the major liver surgery and liver transplantation. The procedures of hepatic vascular inflow occlusion sometimes cause deterioration of the general circulation, and the reperfusion is occasionally followed by a severe shock state. On the other hand, vascular thrombosis of the grafts is a fatal complication in liver transplantation. In such a case, early detection and immediate retransplantation are essential to rescue the patients.The aim of this study is to find a biochemical indicator for early detection of the surgical complications caused by the hepatic vascular inflow occlusion which is often used in major liver surgery or liver transplantation. Plasma samples obtained from the rats with snaring of portal triad were analyzed by high performance liquid chromatography (HPLC) with electrochemical detection. HPLC analysis indicated that the concentration of an unknown substance in the plasma progressively increased with the occlusion time. After the recirculation it increased further and followed by gradual decrease. The unknown substance purified from the plasma was found to have the same HPLC retention time and absorption spectra as uric acid. The substance was therefore identified to be uric acid. During the occlusion for 30 min, the plasma uric acid values were progressively increased from 0.49<plus-minus>0.04 mg/dl to 2.61<plus-minus>0.06 mg/d which was 5-fold higher than the basal values. In contrast, the concentrations of hepatic enzymes such as asparate aminotransferase and lactate dehydrogenase showed almost no change during the occlusThese results suggest that uric acid could be used as a biochemical marker to assess the influence of hepatic vascular inflow occlusion in early stage of liver surgery and to detect possible occurrence of the vascular complication in liver transplantation.

肝血管流入闭塞和完全的肝血管排斥通常被用于主要的肝脏手术和肝移植中。肝血管流入闭塞的程序有时会导致一般循环的恶化，偶尔再灌注后，紧随其后的是严重的休克状态。另一方面，移植物的血管血栓形成是肝移植的致命并发症。在这种情况下，早期检测和立即再移植对于挽救患者至关重要。本研究的目的是找到生化指标，用于早期检测由肝血管流入闭塞引起的手术并发症，这通常用于主要肝脏手术或肝移植。通过高性能液相色谱（HPLC）分析了从大鼠中获得的血浆样品，并通过电化学检测。 HPLC分析表明，血浆中未知物质的浓度随着闭塞时间逐渐增加。再循环后，它进一步增加，然后逐渐减少。发现从血浆纯化的未知物质具有与尿酸相同的HPLC保留时间和吸收光谱。因此，该物质被确定为尿酸。在闭塞30分钟内，血浆尿酸值从0.49 <plus-minus> 0.04 mg/dL逐渐增加到2.61 <plus-minus> 0.06 mg/d，比基础值高5倍。相反，肝氨基转移酶和乳酸脱氢酶等肝酶的浓度几乎没有变化。