Reconstruction of the hepatic tissue exploiting the stem cells of the adult liver.

利用成人肝脏干细胞重建肝组织。

基本信息

批准号：
14370375
负责人：
KOGURE Kimitaka
金额：
$ 7.68万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370375/
关键词：
adult stem cells epithelial stem like cell liver regeneration liver organoid artificial liver regenerative medicine 肝上皮様細胞

项目摘要

The aim of this study is to reconstruct the hepatic tissue from the_adult stem cells of the rat liver. We_already established the method for the isolation of the epithelial stem-like cells from adult rat liver. The epithelial stem-like cells of the rat liver had the features of the stem cells, which had a capacity to renew itself and to give rise to hepatocytes and biliary epithelial cells. These cells also had the capacity to proliferate explosively and to survive for a long term. These cells were examined by histochemistry and iimmunohistochemistry. The results were as follows : Glycogen (+), Afp (+), Albumin (+), Flk-1 (+++), PECOM-1 (+++), von Willbrand Factor (+-), CK7/17 (+-), CD34 (-). Then, we established the method to reconstruct the organoid of liver cells which were isolated from adult rat liver and transplanted into the subcutaneous space of the abdomen of the rat. The average dimension of the hepatic organoid was about 10 mm long and 5 mm width. The histological examination revealed that the hepatic organoid was mainly formed from the liver cells with scattered duct-like structures. The liver cells consisting the hepatic organoid exhibited the weak cytoplasmic AFP immunoreactivity.In summary, this study has shown that the epithelial stem-like cells of the adult rat liver was a good candidate in establishing the functioning hepatic organoid and that the subcutaneous, inguinal fat pad was an appropriate niche in which the transplanted liver cells can give rise to the hepatic organoid.

这项研究的目的是从大鼠肝脏的_ADULT干细胞中重建肝组织。 WE_AlReady建立了从成年大鼠肝脏中分离上皮干细胞的方法。大鼠肝脏的上皮样细胞具有干细胞的特征，该细胞具有更新本身并产生肝细胞和胆道上皮细胞的能力。这些细胞还具有爆炸性并长期生存的能力。这些细胞通过组织化学和IMuno组织化学检查。结果如下：糖原（+），AFP（+），白蛋白（+），FLK-1（+++），PECOM-1（+++），von Willbrand因子（+ - ），CK7/17（+ - ），CD34，CD34（ - ）。然后，我们确定了从成年大鼠肝脏分离并移植到大鼠腹部的皮下空间中分离出的肝细胞器官的方法。肝癌的平均尺寸长约10毫米，宽度为5毫米。组织学检查表明，肝癌主要由具有散射的管道样结构的肝细胞形成。总而言之，这项研究表明，成年大鼠肝脏的上皮干细胞是建立功能功能的肝癌，并且是皮下的，gyuinal fat板可以使the骨上的适当的细胞在thrplant的细胞中，因此，肝癌的肝细胞细胞表现出弱细胞质AFP免疫反应性。