Serum Cytotoxic Factor and its' Inhibitorin Rheumatoid Arthritis.

血清细胞毒性因子及其抑制剂类风湿性关节炎。

• 批准号: 04807106
• 负责人: OCHI Takahiro
• 金额: $ 1.02万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807106/
• 关键词: Reumatoid arthritis; Serum; Citotoxity; Inhibitor; 組織傷害; 細胞傷害性因子

The peripheral blood serum of patients with severe active rheumatoid arthritis (RA) was found to have a cytotoxic effect on autologous mononuclear cells. The cytotoxic factor was partially purified and shown to have a molecular weight of about 900 kd, using gel filtration. The factor differed from cytokines such as tumor necrosis factor-alpha (TNF-alpha) and also differed from complement components. It also differed from hydrolytic enzyme such as protease, collagenase, and phospholipase A_2. It destroyed normal peripheral blood mononuclear cells in vitro at the same concentration as it found in vivo, and also nonspecifically destroyed such human cell lines as CCRF-CEM, Raji, U-937, and HL-60. Each RA patient with this serum cytotoxic factor had severe systemic tissue disease as well as rapid and severe destruction of most joints. Thus, we have identified a cytotoxic factor in RA serum which causes systemic tissue damage. And we also partially pirified the inhibitor against the cytotoxic factor, using gel filtration.

发现严重活动性类风湿关节炎（RA）患者外周血血清对自体单核细胞具有细胞毒性作用。经凝胶过滤，细胞毒性因子部分纯化，分子量约为900 kd。该因子不同于细胞因子，如肿瘤坏死因子- α (tnf - α)，也不同于补体成分。它也不同于蛋白酶、胶原酶、磷脂酶A_2等水解酶。它在体外以与体内相同的浓度破坏正常外周血单个核细胞，并非特异性地破坏CCRF-CEM、Raji、U-937和HL-60等人细胞系。每个患有这种血清细胞毒因子的RA患者都有严重的全身性组织疾病，并且大多数关节都有快速而严重的破坏。因此，我们已经在类风湿性关节炎血清中确定了一种细胞毒性因子，它会导致全身组织损伤。我们也用凝胶过滤，部分提纯了抑制细胞毒性因子的抑制剂。

项目成果

Ochi,T: "Therapeutic Effect of Intradermal Injections with Difucosyl Lactosamine(Dimeric Lex)on Patients with Rheumatoide Arthritis." The Journal of Rheumatology. 20. 2038-2045 (1993)

Ochi,T：“皮内注射二岩藻糖基乳糖胺（二聚 Lex）对类风湿性关节炎患者的治疗效果。”

Hayashida K, Ochi T, Fujimoto M, Owaki H, Shimaoka Y, Ono K, Matsumoto K.: "Bone marrow changes in adjuvant-inducedand collagen-inducedarthritis." Arthritis and Rheumatism. 35. 241-245 (1992)

Hayashida K、Ochi T、Fujimoto M、Owaki H、Shimaoka Y、Ono K、Matsumoto K.：“佐剂诱导和胶原诱导的关节炎中的骨髓变化。”

島岡康則: "慢性関節リウマチ患者に認めた新しい細胞傷害性因子.全身性組織傷害と非特異的細胞傷害性因子" 日整形会誌. 66. 1292 (1992)

Yasunori Shimaoka：“在类风湿性关节炎患者中发现的一种新的细胞毒性因子。全身组织损伤和非特异性细胞毒性因子”日本骨科学会杂志 66. 1292 (1992)。

Fujimoto,M: "Fluctuation of interleukin-1 and-6 activity in bone marrow serum in collagen-induced arthritis in rats." Biomedical Research. 13. 243-251 (1992)

Fujimoto,M：“胶原诱导的大鼠关节炎中骨髓血清中白介素 1 和 6 活性的波动。”

Hayashida,K: "Bone marrow changes in adjuvant-induced and collagen-induced arthritis." Arthritis and Rheumatism. 35. 241-245 (1992)

Hayashida,K：“佐剂诱导和胶原诱导的关节炎中的骨髓变化。”