Abnormal Myeloid Lineage Cells Expressing the Difucosyl or Trifucosyl Type 2 Chain in Bone Marrow Cells of Patinets with Severe Rheumatoid Arthritis.

类风湿性关节炎（RA）患者髂骨髓单个核细胞的绝对数量比非RA对照组增加3倍。相反，在骨骺骨髓或外周血中未观察到显著的MNC增加。在淋巴细胞亚群中，RA患者髂骨髓中HLA-DR+ CD 8+细胞占全部CD 8细胞的百分比显著高于非RA对照组。异常骨髓细胞（CD 14 + CD 15+细胞），具体到严重的RA，被发现更集中在髂骨骨髓比胫骨骨骺骨髓。IL-1 β和GM-CSF可促进这些异常髓系细胞的产生，但T淋巴细胞可抑制这些异常髓系细胞的产生。这些异常的骨髓细胞被证实分化为多形核白细胞。在严重RA患者中，在邻近受影响关节的骨骺骨髓中的MNC和PMN的细胞裂解物中发现高水平的IL-1 α和IL-1 β。IL-6和IL-8在髂骨骨髓血清中检测到显著升高的水平，而在胫骨骨髓和外周血血清中发现低得多的水平。髂骨髓血清IL-6、IL-8水平与滑膜增生程度密切相关。从骨髓和滑膜中建立了基质细胞（护士细胞）系，这些细胞系具有增强的支持前B细胞系和维持异常髓样细胞的能力。

The absolute number of mononuclear cells in the iliac bone marrow was incresed by 3-fold in the rheumatoid arthritis (RA) patients compared with non-RA controls. In contrast, no significant increase of MNC was abserved in the epiphyseal bone marrow or peripheral blood. In lymphocyte subsets, the percentage of HLA-DR+CD8+cells to all CD8 cells in the iliac bone marrow increased significantly in the RA patients compared with the non-RA controls. Abnormal myeloid cells (CD14+CD15+cells) , specific to severe RA,were found to be more concentrated in the iliac bone marrow than in the tibial epiphyseal bone marrow. The production of these abnormal myeloid cells was enhanced by IL-1beta and GM-CSF,but inhibited by T lymphocytes. These abnormal myeloid cells were confirmed to differentiated to polymorphonuclear leukocytes. In patients with severe disease of RA, high levels of IL-1alpha and IL-1beta were found in the cell lysates of MNC and PMN in the epiphyseal bone marrow adjacent to affected joints. Markedly elevated levels of IL-6 and IL-8 were detected in iliac bone marrow serum, and much lower levels were found in the tibial bone marrow and peripheral blood serum. The levels of IL-6 and IL-8 in iliac bone marrow serum showed a close relationship to the extent of synovial proliferation. Stromal cell (nurse cell) line were established from both bone marrow and synovium.These cell lines were shown to have an enhanced ability to support the pre-B-cell lines and to maintain abnormal myeliod cells.