Abnormal Myeloid Lineage Cells Expressing the Difucosyl or Trifucosyl Type 2 Chain in Bone Marrow Cells of Patinets with Severe Rheumatoid Arthritis.

类风湿性关节炎（RA）患者髂骨髓中单个核细胞的绝对数量比非RA对照组增加了3倍。相比之下，骨骺、骨髓和外周血中MNC均未见明显升高。在淋巴细胞亚群中，与非RA对照组相比，RA患者髂骨髓中HLA-DR+CD8+细胞占所有CD8细胞的百分比显著增加。严重类风湿性关节炎特异性异常骨髓细胞（CD14+CD15+细胞）在髂骨髓中比在胫骨骨骺骨髓中更集中。il -1 β和GM-CSF可促进这些异常骨髓细胞的产生，但T淋巴细胞可抑制这些异常骨髓细胞的产生。这些异常的髓样细胞被证实分化为多形核白细胞。在严重RA患者中，在受影响关节附近的骨骺骨髓中MNC和PMN的细胞裂解物中发现高水平的il -1 α和il -1 β。髂骨髓血清IL-6、IL-8水平明显升高，胫骨骨髓及外周血血清IL-6、IL-8水平明显降低。髂骨髓血清IL-6、IL-8水平与滑膜增生程度密切相关。骨髓和滑膜均建立基质细胞（护理细胞）系。这些细胞系被证明具有增强的支持前b细胞系和维持异常髓细胞的能力。

The absolute number of mononuclear cells in the iliac bone marrow was incresed by 3-fold in the rheumatoid arthritis (RA) patients compared with non-RA controls. In contrast, no significant increase of MNC was abserved in the epiphyseal bone marrow or peripheral blood. In lymphocyte subsets, the percentage of HLA-DR+CD8+cells to all CD8 cells in the iliac bone marrow increased significantly in the RA patients compared with the non-RA controls. Abnormal myeloid cells (CD14+CD15+cells) , specific to severe RA,were found to be more concentrated in the iliac bone marrow than in the tibial epiphyseal bone marrow. The production of these abnormal myeloid cells was enhanced by IL-1beta and GM-CSF,but inhibited by T lymphocytes. These abnormal myeloid cells were confirmed to differentiated to polymorphonuclear leukocytes. In patients with severe disease of RA, high levels of IL-1alpha and IL-1beta were found in the cell lysates of MNC and PMN in the epiphyseal bone marrow adjacent to affected joints. Markedly elevated levels of IL-6 and IL-8 were detected in iliac bone marrow serum, and much lower levels were found in the tibial bone marrow and peripheral blood serum. The levels of IL-6 and IL-8 in iliac bone marrow serum showed a close relationship to the extent of synovial proliferation. Stromal cell (nurse cell) line were established from both bone marrow and synovium.These cell lines were shown to have an enhanced ability to support the pre-B-cell lines and to maintain abnormal myeliod cells.