Regulatory effects on the functions of neutrophils by human salivary antimicrobial peptides, histatins

人唾液抗菌肽组蛋白对中性粒细胞功能的调节作用

• 批准号: 04807132
• 负责人: SUGIYAMA Katsumi
• 金额: $ 1.28万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807132/
• 关键词: Human saliva; Histatins; Assay; HPLC; H_2O_2; Neutrophil; Diurnal changes; 多形核白血球; 過酸化水素; 遊走能; 貪食能; 唾液内濃度; 日内変動

1. Quantitative determination of histatins and their characterization.The assay method for histatins in human saliva using a reversed-phase high performance liquid chromatography has been developed. The histatins have been bound to the high-molecular mass acid components in saliva. A mean value of the total histatins in whole saliva from healthy subjects was 28.6 muM and sex related differences for the histatin concentration was not observed. Diurnal changes of the histatin secretion did not show rhythms. Histatins in the saliva were autodegraded by proteolytic enzymes present in the saliva, as incubated at pH 7.0. In secreted saliva, histatin 5 was not generated from histatin 3.2. Inhibition by histatins on H_2O_2 generation from neutrophils.Neutrophils generated H_2O_2 in the addition of fMLP on the fibronectin-coated dish plate and this H_2O_2 production was inhibited by histatins dose-dependently, PMA-induced H_2O_2 generation was not inhibited by them. Histatins could inhibit the H_2O_2 generation via receptors on the neutrophils. On the other hand, histatins did not inhibit their chemotaxis and phagocytosis.These results suggest that antimicrobial histatins are secreted into the oral cavity constantly, and protect the oral mucous membrane from active oxygen generated by neutrophils.

1.组抑素的定量测定及其性质建立了人唾液中组抑素的反相高效液相色谱法。组抑素与唾液中的高分子酸组分结合在一起。健康受试者唾液中总组抑素的平均值为28.6微米，且未观察到性别差异。组蛋白分泌的日变化无节律。唾液中的组蛋白被唾液中存在的蛋白水解酶在pH 7.0下孵育时自动降解。在分泌的唾液中，组蛋白5不是由组蛋白3.2产生的。组他汀类药物对中性粒细胞产生H_2O_2的抑制作用：中性粒细胞在纤维连接蛋白包被的培养皿上加入fMLP后产生H_2O_2，这种H_2O_2产生被组他汀类药物剂量依赖性地抑制，但不能抑制PMA诱导的H_2O_2产生。组素可通过中性粒细胞表面受体抑制H_2O_2的产生。另一方面，组他汀类药物并不能抑制其趋化和吞噬功能。这些结果表明，抗菌剂组他汀类药物持续分泌到口腔中，保护口腔粘膜免受中性粒细胞产生的活性氧的影响。

项目成果

Sugiyama, Katsumi: "Anti-lipopolysaccharide activity of histatins, peptides from human saliva." Experientia. 49. 1095-1097 (1993)

Sugiyama，Katsumi：“组氨酸的抗脂多糖活性，来自人类唾液的肽。”

Sugiyama Katsumi: "High-performance liquid chromatographic determination of histatins in human saliva" J.Chromatography. 619. 306-309 (1993)

Sugiyama Katsumi：“人唾液中组氨酸的高效液相色谱测定”J.Chromatography。

Sugiyama Katsumi: "Anti-lipopolysaccharide activity of histatins,peptides from human saliva" Experientia. 49. 1095-1097 (1993)

Sugiyama Katsumi：“来自人类唾液的组氨酸、肽的抗脂多糖活性”实验。

Sugiyama, Katsumi: "High-performance liquid chromatographic determination of histatins in human saliva." J.Chromatography. 619. 306-309 (1993)

Sugiyama, Katsumi：“高效液相色谱法测定人类唾液中的组蛋白。”