Studies on histidine-rich polypeptides (histatins) in human saliva

1.一个简单而快速的孤立的人类血统。来自丰富的历史性多肽（组蛋白1、3和5）的人类全人类都是由肝素-环凝胶和反向高性能液体色谱（hplc）上的亲和力色谱法隔离的。约6毫克的组织混合物从100毫升的Saliva中提取。历史1、3和5包括38、32和24氨基酸居住地，重量为4929、4063和3037，对此有相当大的分子重量。他们在分子中包含了7种历史学的存在。历史学的释放是由历史学引起的。Histatin 3和5在浓度范围内10-40米的孤立大鼠肥大细胞中释放出与剂量有关的Histatin 3和5，在浓度范围内释放出相应的Histatin 1。这篇文章的释放取决于温度，并在37 ° C时完成了10秒。这一关系不符合从肥大细胞中提取的乳酸脱氢酶的泄漏。所以，这是因为历史导致了历史的释放，这是由一种外在机制引起的。抗内毒素对历史的影响。（1）抑制内毒素（LPS）对极限反应的凝胶的历史学。（2） Histatins减少了LPS的补充诱导性血细胞的抑制。（3）在琼脂糖凝胶中形成了几种残留脂蛋白的历史。

1. A simple and rapid isolation of histatins from human saliva. Histidine-rich polypeptides (histatin 1,3 and 5) from human whole saliva were isolated by affinity chromatography on heparine-ultrogel and reverse-phase high performance liquid chromatography (hplc). About 6 mg of the mixture of histatins were obtained from 100 ml of the saliva. Histatins 1,3 and 5 contained 38, 32 and 24 amino acid residues, and had molecular weight of 4929, 4063, and 3037, respectively. They contained 7 residues of histidine in their molecules.2. Histamine release induced by histatins. Histatin 3 and 5 produced dose-related histamine release from isolated rat mast cells in the concentration range of 10-40 m, respectively, and also histatin 1 ded in the range 40 - 100 m. This histamine release was temperature dependent and completed within 10 sec at 37゜c. This relase was not accompanied by the leakage of lactate dehydrogenase from mast cells. Thus, it was suggested that the histatins caused the release of histamine by an exocytotic mechanism.3. Anti-endotoxin effect of histations. 1) Histatins inhibited the gelation by endotoxin (LPS) on limulus reaction. 2) Histatins reduced the inhibition by LPS of complement-induced hemolysis. 3) histatins formed the precipitates with several lps in the agarose gel.