Defensive action in oral cavity of human salivary peptide, histatins

人唾液肽、组氨酸在口腔中的防御作用

• 批准号: 07672015
• 负责人: SUGIYAMA Katsumi
• 金额: $ 1.66万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672015/
• 关键词: Human saliva; Histatins; Lysozyme; LPS; LTA; Bacterial aggregation; Bacterial exclusion; リポ多糖体; リポタイコ酸; 好中球; ス-パオキサイド

1. Characterization of human salivary histatinsHuman salivary histatins 1 and 3 were coded by HIS 1 and HIS 2. But proof of gene which code histatin 5 is still lacking. I demonstrated that histatin 5 was produced by alpha-chymotrypsin from histatin 3 in vitro.2. Effects of histatins on lipoteichoic acid (LTA)Histatins were directly bound to LTA which was constituent of gram-positive bacterial cell wall. They were found to neutrarize for the LTA-induced various biological activities.3. A rapid purification of lysozymeI developed a rapid and simple method of the purification of lysozyme from human mixed saliva using heparin-affinity chromatography and high-performance liquid chromatography. Molecular weight of salivary lysozyme was 14,690, and its activity was three-fold higher than that of egg-white lysozyme.4. Bacterial aggregation induced by histatins and its enhancement of lysozymeI found that salivary histatins induced bacterial aggregation, and lysozyme enhanced the aggregation. These findings suggest that salivary lysozyme may facilitate the clerance of bacteria from the oral cavity by means of the aggregation with histatins.

1.人唾液组胺素的性质人唾液组胺素1和3分别由HIS 1和HIS 2编码.但目前尚缺乏编码histatin 5基因的证据。我证明了histatin 5是由α-糜蛋白酶在体外从histatin 3产生的。组胺素对脂磷壁酸（LTA）的影响组胺素与革兰氏阳性菌细胞壁的组成成分LTA直接结合。发现它们可以中和LTA诱导的各种生物活性。3.一种快速纯化溶菌酶的方法我建立了一种从人混合唾液中快速、简便地纯化溶菌酶的方法。唾液溶菌酶的分子量为14，690，其活性是蛋清溶菌酶的3倍.组胺素诱导细菌聚集及其对溶菌酶的增强作用我发现唾液组胺素诱导细菌聚集，而溶菌酶增强细菌聚集。提示唾液溶菌酶可能通过与组胺素的聚集作用促进细菌从口腔中清除。

项目成果

杉山勝三: "ヒト唾液リゾチームの迅速精製とヒスタチンの細菌凝集に対する増強作用" 日本唾液腺学会誌. 38. 36-37 (1997)

Katsuzo Sugiyama：“人唾液溶菌酶的快速纯化和组蛋白对细菌聚集的增强作用”日本唾液腺学会杂志 38. 36-37 (1997)。